产品名称：人扁桃体内皮细胞

收到人扁桃体内皮细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

人扁桃体内皮细胞The tonsils are part of the lymphatic system and are located in the pharynx. The tonsils are the immune system’s first defense against foreign pathogens and help to prevent respiratory and gastrointestinal infections. Tonsil endothelial cells (TEC) line the blood vessels and actively participate in fluid balance, cellular transport, and immunity [1]. TEC also perform specialized functions for the tonsils including recruiting lymphocytes from the blood [2, 3]. Studies have shown that TEC express proteins that aid in lymphocyte adhesion and extravasation [3]. Morphological and physiological changes in human TEC (HTEC) can lead to the development of tonsillitis. HTEC are a useful model for studying inflammation in the tonsils.

HTEC from ScienCell Research Laboratories are isolated from human tonsil. HTEC are cryopreserved at passage one and delivered frozen.  Each vial contains >5 x 10^5 cells in 1 ml volume. HTEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM). HTEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HTEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.

Catalog No. 2550

Country of Manufacture United States

Product Code HTEC

Size/Quantity >5 x 105 cells in 1 ml volume.

Product Use HTEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Shipping Info Dry ice.

References [1] Kelley P, Tempero R. (2010) "Lymphatic vessel hypertrophy in in inflamed human tonsils." Lymphat Res Biol. 8(2): 121-126.

[2] Garrafa E, Alessandri G, Benetti A, Turetta D, Corradi A, Cantoni A, Cervi E, Bonardelli S, Parati E, Giulini S, Ensoli B, Caruso A. (2006) "Isolation and characterization of lymphatic microvascular endothelial cells from human tonsils". J Cell Physiol. 207(1): 107-113.

[3] Palmeri D, Zuo F, Rosen S, Hemmerich S. (2004) "Differential gene expression profile of human tonsil high endothelial cells: implications for lymphocyte trafficking." J Leukoc Biol. 75(5): 910-927.

人扁桃体内皮细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，人扁桃体内皮细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
人扁桃体内皮细胞其它细胞系展示表：yb1164 RT4（膀胱癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb1165 J82（膀胱癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb1166 SHG44（胶质瘤细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb1167 LoVo（人结肠癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb1168 V79(中国仓鼠肺细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb1169 U937（组织细胞**瘤）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb1170 PT-67（病毒转染小鼠细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb1171 HEL（红白血病细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb1172 293（转染腺病毒E1A基因的人肾上皮细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb1173 C6（脑胶质瘤细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb1174 SHZ-88(大鼠乳腺癌细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb1175 CBRH－7919（大鼠肝癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb1176 NCL-H460（非小细胞肺癌）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb1177 F9（畸胎瘤细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb1178 5637（人膀胱癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb1179 A549（人肺腺癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb1180 MCF-7(人乳腺癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb1181 B16（小鼠黑色素瘤细胞） 品牌ATCC 规格25毫升/株. 货期1-2周
yb-2327. HCC1428（人乳腺癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-45. A-704(人肾癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-5892. NCI-H1755 [H1755]（人肺癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-52. SK-HEP-1（人肝癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb5844. NCI-H838 [H838]（人肺癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周