产品名称：人宫颈上皮细胞

收到人宫颈上皮细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

人宫颈上皮细胞The cervix is the lower portion of the uterus and functions to promote fertility and protect the female reproductive tract and fetus from potential pathogens. In addition, the cervix plays a critical role in childbirth by effacing and dilating to allow the baby to pass out of the uterus [1]. Cervical epithelial cells (CerEpiC), which line the cervix, maintain structural integrity and limit the passage of molecular and cellular substances into the cervix using intercellular junctions [2, 3]. Studies have shown that patients infected with human papillomavirus (HPV) causes inflammation of the epithelial cell layer which can lead to the development of cervical carcinoma [4]. Primary human CerEpiC (HCerEpiC) are a useful in vitro model for studying the pathophysiology of cervical polyps, HPV, and cervical cancer.

HCerEpiC from ScienCell Research Laboratories are isolated from human uterus. HCerEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCerEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18. HCerEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HCerEpiC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Cervical Epithelial Cell Medium (CerEpiCM, Cat. #7061) for culturing HCerEpiC in vitro.

人宫颈上皮细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，人宫颈上皮细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
人宫颈上皮细胞其它细胞系展示表：yb-7744. TE 115.T（人软组织纤维瘤细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-104. Cates-1B(人睾丸**胚胎性癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-2233. SNU-398（人肝癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-8064. Hep 3B2.1-7 [Hep 3B; Hep-3B; Hep3B]（人肝癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-2235. SNU-182(人肝癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-8024. PLC/PRF/5（人肝癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-169. WERI-Rb-1（人视网膜母细胞瘤）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1572. PA-1(人卵巢畸胎瘤细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-126. Hs 578T（人乳腺成纤维细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-125. Hs 578Bst（人乳腺成纤维细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-7762. TE 354.T(人皮肤基底细胞癌）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-7761. TE 353.Sk(人正常皮肤细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-228. SW480 [SW-480]（人结肠癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-227. SW620 [SW-620]（人结肠腺癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-5803. NCI-H1299（人肺癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1721. PC-12（大鼠肾上腺嗜铬细胞瘤）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1651. COS-7（非洲青猴肾细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-11731. TOV-112D（人上皮性卵巢癌细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-171. MRC-5(人正常胚肺成纤维细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周
yb-5875. NCI-H1563 [H1563]（人胚肺细胞）. 品牌ATCC 规格25毫升/株. 货期1-2周