产品名称：人乳腺微血管内皮细胞

收到人乳腺微血管内皮细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

人乳腺微血管内皮细胞Description

Vascular endothelial cells line the blood vessels and contribute to many biological processes such as hematosis, coagulation, trafficking of lymphocytes, and the inflammatory response. The endothelial cell is of primary importance in the extravasation of leukocytes from the circulation into sites of tissue inflammation. In the case of mastitis, the magnitude and duration of inflammation can be related to such parameters as endothelial adhesion molecules expression, cytokine secretion, as well as changes in vascular permeability [1]. Histological study demonstrates that mammary microvasculature is a prominent feature of intralobular tissue [2] and the endothelial cells play an important role in the study of mastitis and associated mammary gland immunopathology [3].

HMMEC from ScienCell Research Laboratories are isolated from human breast tissue. HMMEC are cryopreserved after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HMMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HMMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HMMEC are guaranteed to further expand for 15 population doublings at the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HMMEC.

Product Use

HMMEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1] KM. Ahem, MR. Davis & IM. Sordillo (1995) Isolation and characterization of bovine mammary endothelial cells. Method in Cell Science. 17(1):41-46.

[2] AG. Naccarato, P. Viacava, G. Bocci, G. Fanelli, P. Aretini, A. Lonobile, G. Montruccoli and G. Bevilacqua (2003) Definition of the microvascular pattern of the normal human ***** mammary gland. J. Anat. 203:599-603.

[3] V. Sigurdsson, AJR. Fridriksdottir. J. Kjartansson, JG. Jonasson, M. Steinarsdottir, OW. Petersen, HM. Ogmundsdottir, and T Gudjsonsson (2006) Human breast microvascular endothelial cells retain phenotypic traits in long-ter finite life span culture. In Vitro Cellular & Developmental Biology - Animal 42(10):332-340.

人乳腺微血管内皮细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，人乳腺微血管内皮细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
人乳腺微血管内皮细胞其它细胞系展示表：yb-1223 RF/6A(猴脉络膜-视网膜(内皮)细胞 ). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1224 MDBK (NBL-1)(牛肾细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1225 LLC-MK2(恒河猴肾细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1226 B95-8(EBV 转化的绒猴白细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1227 Vero(非洲绿猴肾细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1228 COS-7(SV40 转化的非洲绿猴肾细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1229 CV-1(非洲绿猴肾细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1230 COS-1(SV40 转化的非洲绿猴肾细胞 ). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1231 293 [HEK-293] (人胚肾细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1232 HBL-100(整合SV40 基因的乳腺上皮细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1233 CGM1(EB 病毒转化的B 细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1234 WISH(羊膜细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1235 HL-7702(肝细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1236 Ha Fe(羊膜细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1237 QSG-7701(肝细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1238 HFL-I(MEMα)(胚肺成纤维细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1239 HeLa [ Chang Liver ](张氏肝细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1240 V79-4(中国仓鼠肺细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1241 CHL/IU [ CHL-11 ](中国仓鼠肺细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1242 CHO(中国仓鼠卵巢细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1243 BHK(幼仓鼠肾细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1244 R 1610(中国仓鼠体细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1245 BRL 3A(大鼠肝细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1246 NRK(大鼠肾细胞). 品牌ATCC 规格25毫升/株. 货期1-2周
yb-1247 L929(成纤维细胞). 品牌ATCC 规格25毫升/株. 货期1-2周