产品名称：人肺间充质干细胞

收到人肺间充质干细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

人肺间充质干细胞Description

Mesenchymal stem cells (MSC) are a well-characterized population of ***** stem cells. MSC have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. This property, in combination with MSC’s developmental plasticity, has generated tremendous interest in the potential use of MSC to replace damaged tissues. Fetal lung has been identified as a rich source of MSCs and has been reported that MSCs can differentiate into neural cells in addition to their mesenchymal differentiation potential [1]; enhance the engraftment of human umbilical cord blood-derived CD34 hematopoietic cells in nonobese diabetic-severe combined immunodeficiency mice [2]. Flow cytometric analysis showed that fetal lung MSCs expressed CD13, CD29, CD44, CD90, CD10^5, CD166, and HLA-ABC.

HPMSC from ScienCell Research Laboratories are isolated from lung tissue. HPMSC are cryopreserved at passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HPMSC are characterized by immunofluorescent method with antibodies to CD73, CD90 and CD105. HPMSC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPMSC are guaranteed to further culture at the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. No. 7501) for the culturing of HPMSC in vitro.

Product Use

HPMSC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

Reference

[1]. Fan CG, Tang FW, Zhang QJ, Lu SH, Liu HY, Zhao ZM, Liu B, Han ZB, Han ZC (2005) Characterization and neural differentiation of fetal lung mesenchymal stem cells. Cell Transplant. 14:311-321.

[2]. Noort WA, Kruisselbrink AB, in 't Anker PS, et al. (2002) Mesenchymal stem cells promote engraftment of human umbilical cord blood-derived CD34(+) cells in NOD/SCID mice. Exp. Hematol. 30:870-878.