产品名称：小鼠心肌细胞

收到小鼠心肌细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠心肌细胞Description

The cardiac myocyte is the most physically energetic cell in the body. Its contraction is myogenic, i.e. it is independent of nervous stimulation. All cardiac myocytes are capable of spontaneous rhythmic depolarization and repolarization of their membrane. Cardiac myocytes occupy as much as 75% of cardiac mass but constitute only about one third of the total cell number in the heart. They are highly specialized high-oxygen-content cells and house a large number of mitochondria [1]. Differentiated cardiac myocytes have little capacity to proliferate and show the hypertrophic growth in response to alpha1-adrenergic stimuli via the Ras/MEK pathway [2]. Cardiac myocyte hypertrophy and apoptosis have been implicated in the loss of contractile function during heart failure. Cardiac myocytes have a complex network of signals that regulates their essential role in the rhythmic pumping of the heart [3]. This network is an appealing model system in which to study the basic principles of cellular signaling mechanisms leading to cardiac myocyte death.

MCM from ScienCell Research Laboratories are isolated from postnatal day 2 mouse heart. MCM are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. MCM are characterized by immunofluorescence with antibodies specific to smooth muscle actin, sacromeric alpha-actinin, and tropomyosin. MCM are negative for mycoplasma, bacteria, yeast, and fungi. MCM are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, MCM are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.

Recommended Medium

It is recommended to use Cardiac Myocyte Medium (CMM, Cat. No. 6201) for the culturing of MCM in vitro.

Product Use

MCM are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1] Bodyak, N., Kang, P. M., Hiromura, M., Sulijoadikusumo, I., Horikoshi, N., Khrapko, K. and Usheva, A. (2002) Gene expression profiling of the aging mouse cardiac myocytes. Nucleic Acids Research 30(17):3788-3794.

[2] Tamamori-Adachi, M., Ito, H., Nobori, K., Hayashida, K., Kawauchi, J., Adachi, S., Ikeda, M. A. and Kitajima, S. (2002) Expression of cyclin D1 and CDK4 causes hypertrophic growth of cardiomyocytes in culture: a possible implication for cardiac hypertrophy. Biochem Biophys Res Commun 296(2):274-80.

[3] Sambrano, G.R., Fraser, I., Han, H., Ni, Y., O'Connell, T., Yan, Z. and Stull, J. T. (2002) Navigating the signaling network in mouse cardiac myocytes. Nature 420(6916):712-4.

小鼠心肌细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，小鼠心肌细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
小鼠心肌细胞其它细胞系展示表：CoLo  320DM 结肠癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HEC-1B **内膜癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HCT-8 回盲肠腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
JEG-3 绒癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HCT-116 低分化结肠腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
DU-145 前列腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HT-29 结肠腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
LNCa 前列腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Lovo 结肠癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
LNCaP ybone FGC 前列腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SW480 结肠癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
PC-3 前列腺癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
BEL-7402 肝细胞癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Tsu-Pr1 非雄**依赖型前列腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
HCC-9204 肝癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
HOS 骨肉瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Hep3B 肝癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
MG-63 成骨肉瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Hep G2 肝癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
Saos-2 骨肉瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
SMMC-7721 肝癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周
U-2 OS 骨肉瘤细胞 品牌ATCC 规格25毫升/株. 货期1-2周
PAN-1 胰腺导管上皮癌细胞 品牌ATCC 规格25毫升/株. 货期1-2周