产品名称：小鼠**成纤维细胞

收到小鼠**成纤维细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠**成纤维细胞Description

Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen [1]. There is evidence showing that fibroblasts in different organs are intrinsically different [2]. In lymphoid compartment, fibroblasts construct a unique conduit system with intriguing size exclusion which plays a fundamental role in regulating immune response and homeostasis of tissue fluids [3].

MLF from ScienCell Research Laboratories are isolated from mouse lymph node. MLF are cryopreserved as primary cultures and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. MLF are characterized by their spindle-shaped morphology and immunofluorescence with antibody specific to fibronectin. MLF are negative for mycoplasma, bacteria, yeast, and fungi. MLF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing MLF in vitro.

Product Use

MLF are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Shipping

Dry ice.

Reference

[1] Gabbiani G, Rungger-Brandle E. (1981) “The fibroblast.” In Glynn LE, Handbook of Inflammation, Vol. 3: Tissue Repair and Regeneration (pp 1-50). Amsterdam: Elsevier.

[2] Conrad GW, Hart GW, Chen Y. (1977) “Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks.” J Cell Sci. 26: 119-37.

[3] Roozendaal R, Mebius RE, Kraal G. (2008) “The conduit system of the lymph node.” Int Immunol. 20: 1483-7.

小鼠**成纤维细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，小鼠**成纤维细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
小鼠**成纤维细胞其它细胞系展示表：B16 黑色素瘤 品牌ATCC 规格25毫升/株. 货期1-2周
J774A.1 单核细胞－巨噬细胞 品牌ATCC 规格25毫升/株. 货期1-2周
C127 乳腺肿瘤 品牌ATCC 规格25毫升/株. 货期1-2周
RAW264.7 单核细胞－巨噬细胞 品牌ATCC 规格25毫升/株. 货期1-2周
F9 胚胎瘤 品牌ATCC 规格25毫升/株. 货期1-2周
NG108-15 小鼠神经细胞瘤×大鼠神经胶质细胞杂交细胞 品牌ATCC 规格25毫升/株. 货期1-2周
C6 胶质瘤 品牌ATCC 规格25毫升/株. 货期1-2周
RH－35 肝癌 品牌ATCC 规格25毫升/株. 货期1-2周
SHZ－88 乳腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
CBRH－7919 肝癌 品牌ATCC 规格25毫升/株. 货期1-2周
PC-12 肾上腺嗜铬细胞瘤 品牌ATCC 规格25毫升/株. 货期1-2周
A431 表皮癌 品牌ATCC 规格25毫升/株. 货期1-2周
QGY-7701 肝癌 品牌ATCC 规格25毫升/株. 货期1-2周
A673 横纹肌癌 品牌ATCC 规格25毫升/株. 货期1-2周
QGY-7703 肝癌 品牌ATCC 规格25毫升/株. 货期1-2周
Tca-8113 舌鳞癌 品牌ATCC 规格25毫升/株. 货期1-2周
SMMC-7721 肝癌 品牌ATCC 规格25毫升/株. 货期1-2周
Acc-2 涎腺腺样囊性癌 品牌ATCC 规格25毫升/株. 货期1-2周
786-O 肾透明细胞腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
Acc-3 涎腺腺样囊性癌 品牌ATCC 规格25毫升/株. 货期1-2周
PC-3 前列腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
KB 口腔表皮样癌 品牌ATCC 规格25毫升/株. 货期1-2周
T-24 膀胱变移细胞癌 品牌ATCC 规格25毫升/株. 货期1-2周
HEp-2 喉表皮样癌 品牌ATCC 规格25毫升/株. 货期1-2周
SCaBER 膀胱鳞癌 品牌ATCC 规格25毫升/株. 货期1-2周