产品名称：小鼠中脑星形胶质细胞

收到小鼠中脑星形胶质细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠中脑星形胶质细胞Description

Astrocytes are the major cell type in the mammalian brain. They provide a variety of supportive functions for their partner neurons in the central nervous system, such as neuronal guidance during development, ion and water homeostasis, blood flow regulation, neurotransmission, energy metabolism, and immune defense [1]. Astrocytes have also been implicated in various pathological processes [2]. Impairment of normal astrocyte functions during stroke and other insults can critically influence neuron survival. Long-term recovery after brain injury, through neurite outgrowth, synaptic plasticity, or neuron regeneration, is also influenced by astrocyte surface molecule expression and trophic factor release [3]. Numerous studies have demonstrated that astrocytes are among the most functionally diverse group of cells in the CNS [4]. Cultured mouse astrocytes are a useful in vitro model for studying the molecular and cellular properties of the central nervous system.

MA-mb from ScienCell Research Laboratories are isolated from embryonic day 15 mouse midbrain. MA-mb are cryopreserved as primary cultures and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MA-mb are characterized by immunofluorescence with antibody specific to GFAP. MA-mb are negative for mycoplasma, bacteria, yeast, and fungi. MA-mb are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Astrocyte Medium-animal (AM-a, Cat. #1831) for culturing MA-mb in vitro.

Product Use

MA-mb are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Reference

[1] Oberheim N, Goldman S, Nedergaard M. (2012) "Heterogeneity of astrocytic form and function." Methods in Mol Biol. 814: 23-45.

[2] van der Laan LJ, De Groot CJ, Elices MJ, Dijkstra CD. (1997) "Extracellular matrix proteins expressed by human ***** astrocytes in vivo and in vitro: an astrocyte surface protein containing the CS1 domain contributes to binding of lymphoblasts." J Neurosci Res. 50: 539-48.

[3] Chen Y, Swanson RA. (2003) "Astrocytes and brain injury." J Cereb Blood Flow Metab. 23: 137-49. [4] Shao Y, McCarthy KD. (1994) "Plasticity of astrocytes." Glia. 11: 147-55.