产品名称：CD1小鼠脑血管周细胞

收到CD1小鼠脑血管周细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

CD1小鼠脑血管周细胞Description

Brain Vascular Pericytes (BVP) are perivascular cells that are closely associated with the endothelium of capillaries and other small vessels [1]. BVP, located between endothelial cells and astrocytes in the brain, communicate with other cells by extending long cytoplasmic processes which wrap around the capillaries [1, 2]. BVP participate in a variety of processes including angiogenesis, endothelial cell survival, regulation of capillary blood flow, and establishment and maintenance of the blood-brain barrier [3, 4]. Pericyte dysregulation has been linked to several pathological conditions such as hypertension, diabetic retinopathy, atherosclerosis, multiple sclerosis, Alzheimer’s disease, and tumor angiogenesis [2, 4]. The unique and diverse functions of BVP make them novel candidates for cell therapy in regenerative medicine. Cultured primary mouse BVP (MBVP) are a useful in vitro model for understanding the molecular mechanisms of blood-brain barrier regulation and for studying a wide variety of central nervous system diseases.

MBVP from ScienCell Research Laboratories are isolated from ***** mouse brain. MBVP are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MBVP are characterized by immunofluorescence with antibody specific to Neural/glial antigen 2 (NG2) and α-smooth muscle actin. MBVP are negative for mycoplasma, bacteria, yeast, and fungi. MBVP are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Pericyte Medium (PM, Cat. #1201) for culturing MBVP in vitro.

Product Use

MBVP are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Shipping

Dry ice.

Reference

[1] Dore-Duffy P, Cleary K. (2011) “Morphology and properties of pericytes.” Methods Mol Biol. 686:49-68.

[2] Allt G, Lawrenson JG. (2001) “Pericytes: cell biology and pathology.” Cells Tissues Organs. 169: 1-11.

[3] Daneman R, Zhou L, Kebede A, Barres B. (2010) “Pericytes are required for blood-brain barrier integrity during embryogenesis.” Nature. 468:562-566. [4] Kutcher M, Herman I. (2009) “The pericyte: cellular regulator of microvascular blood flow.” Microvasc Res. 77: 235-246.

CD1小鼠脑血管周细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，CD1小鼠脑血管周细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
CD1小鼠脑血管周细胞其它细胞系展示表：yb-hxbz-443 人黑色素瘤细胞 HME2 规格25毫升/株.
yb-hxbz-444 人黑色素瘤细胞 HS-6 规格25毫升/株.
yb-hxbz-445 人黑色素瘤细胞 MV3   规格25毫升/株.
yb-hxbz-446 人黑色素瘤细胞 HSC1 规格25毫升/株.
yb-hxbz-447 人黑色素瘤细胞 NW38 规格25毫升/株.
yb-hxbz-448 人黑色素瘤细胞 B16 规格25毫升/株.
yb-hxbz-449 人黑色素瘤细胞 SK23 规格25毫升/株.
yb-hxbz-450 人黑色素瘤细胞 SK37 规格25毫升/株.
yb-hxbz-451 人黑色素瘤细胞 A875 规格25毫升/株.
yb-hxbz-452 人皮肤黑色素瘤细胞 SK-MEL-1 规格25毫升/株.
yb-hxbz-453 人恶性黑色素瘤细胞 A375 规格25毫升/株.
yb-hxbz-454 人侵袭性脉络膜黑色素瘤细胞 M619 规格25毫升/株.
yb-hxbz-455 人黑色素瘤细胞 M14 规格25毫升/株.
yb-hxbz-456 人葡萄膜黑色素细胞 UM 规格25毫升/株.
yb-hxbz-457 人视网膜色素上皮细胞 D407 规格25毫升/株.
yb-hxbz-458 人视网膜母细胞瘤 Rb 规格25毫升/株.
yb-hxbz-459 人眼脉络黑色素瘤细胞 OCM-1 规格25毫升/株.
yb-hxbz-460 人眼脉络黑色素瘤细胞 MuM－2C   规格25毫升/株.
yb-hxbz-461 人眼脉络黑色素瘤细胞 MuM－2B 规格25毫升/株.
yb-hxbz-462 人低侵袭性脉络膜黑色素素瘤细胞 OCM-1A 规格25毫升/株.
yb-hxbz-463 人视网膜母细胞瘤 Y79 规格25毫升/株.
yb-hxbz-464 荧光素酶转染人成骨肉瘤细胞 U2OS-Luc teT-on 规格25毫升/株.
yb-hxbz-465 人横纹肌肉瘤细胞 TE671 subline No.2 规格25毫升/株.
yb-hxbz-466 人眼脉络黑色素瘤细胞 C918 规格25毫升/株.
yb-hxbz-467 人横纹癌细胞 A673   规格25毫升/株.
yb-hxbz-468 人恶性胚胎横纹肌瘤细胞 RD 规格25毫升/株.
yb-hxbz-469 人表皮癌细胞 A-431 规格25毫升/株.
yb-hxbz-470 人正常皮肤细胞 HaCaT 规格25毫升/株.