产品名称：小鼠**单核细胞

收到小鼠**单核细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠**单核细胞Lymphatic Mononuclear Cells (LMC) are located in the lymph nodes and perform vital functions for the innate and adaptive immune system [1]. Primary LMC are a mixed population of single nucleus cells which include T-cells (helper T-cells, cytotoxic T-cells, gamma delta T-cells), Bcells, and natural killer (NK) cells. Primary mouse LMC (MLMC) can be used to study the innate and adaptive immune system.

MLMC from ScienCell Research Laboratories are isolated from healthy ***** C57BL/6 mouse lymph nodes. MLMC are depleted of erythrocytes, cryopreserved immediately after isolation, and delivered frozen. MLMC are a mixed population of cells that include T-cells, B-cells, and NK cells. Each vial contains at least 10 million cells in 1 ml volume. MLMC are quality control tested for viability. MLMC are negative for mycoplasma, bacteria, yeast, and fungi. MLMC can be maintained for a limited period of time in culture using the conditions provided by ScienCell Research Laboratories and are not intended for long-term culture.

Catalog No. M2540-57

Country of Manufacture United States

Product Code MLMC

Size/Quantity 10 million cells in 1 ml volume

Product Use MLMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Shipping Info Dry ice.

References [1] Willard-Mack C. (2006) "Normal structure, function, and histology of lymph nodes." Toxicol Pathol 34(