产品名称：小鼠肺成纤维细胞

收到小鼠肺成纤维细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠肺成纤维细胞Description

The most abundant cell type in the lung interstitium is the fibroblast. These fibroblasts resemble ordinary fibroblasts, but also possess some distinguishing features, such as long, branching processes and gap junctions. Pulmonary fibroblasts function to produce type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa. They play an important role in the repair and remodeling processes following tissue damage. The controlled accumulation of fibroblasts to sites of inflammation is crucial to effective tissue repair after injury [1]. An inadequate or an excessive accumulation of fibroblasts can result in abnormal tissue function. For example, excess proliferation of fibroblasts contributes to adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension [2].

MPF from ScienCell Research Laboratories are isolated from postnatal day 2 C57BL/6 mouse lung. MPF are cryopreserved at passage 1 and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MPF are characterized by immunofluorescence with antibody specific to fibronectin. MPF are negative for mycoplasma, bacteria, yeast, and fungi. HPF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing MPF in vitro.

Product Use

MPF are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Shipping

Dry ice.

Reference

[1] Kuwano K, Hagimoto N, Hara N. (2001) “Molecular mechanisms of pulmonary fibrosis and current treatment.” Curr Mol Med. 1: 551-73.

[2] Das M, Dempsey EC, Reeves JT, Stenmark KR. (2002) “Selective expansion of fibroblast subpopulations from pulmonary artery adventitia in response to hypoxia.” Am J Physiol Lung Cell Mol Physiol. 282: L976-86.