产品名称：小鼠成纤维细胞束膜

收到小鼠成纤维细胞束膜时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠成纤维细胞束膜Description

Perineurial fibroblasts are of mesenchymal origin and form the perineurium. The perineurium plays an important role in maintaining the integrity of the internal peripheral nerve environment by creating a physical barrier that, under physiologic conditions, limits the entry of biologically active proteins, infectious agents, and blood-borne cells into the nerve bundles [1]. The perineurial fibroblasts are characterized by distinct ultrastructural features, including non-branching thin cytoplasmic processes coated by an external lamina and joined at their ends by a tight junction, few organelles, actin and vimentin filaments, and numerous pinocytotic vesicles [2]. Perineurial fibroblasts are initially recruited from the surrounding mesenchyme to form a loose, permeable sheath around axons and Schwann cells, where they are separated by the extracellular matrix. These cells later undergo a mesenchymal-to-epithelial transition to form tight junctions and organize into the perineurium. Perineurial fibroblasts are immunoreactive for vimentin and epithelial membrane antigen but not for the Schwann cell marker S-100 [3].

MPNF from ScienCell Research Laboratories are isolated from postnatal day 8 C57BL/6 mouse sciatic nerve. MPNF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MPNF are characterized by immunofluorescence with antibodies specific to vimentin, S-100, GFAP and CD90. MPNF are negative for mycoplasma, bacteria, yeast, and fungi. MPNF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing MPNF in vitro.

Product Use

MPNF are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Shipping

Dry ice.

Reference

[1] Salzer JL. (1999) “Creating barriers: a new role for Schwann cells and desert hedgehog.” Neuron. 22: 627-9.

[2] Erlandson RA. (1991) “The enigmatic perineurial cell and its participation in tumors and in tumor like entities.” Ultrastruct Pathol. 15: 335-51.

[3] Ariza A, Bilbao JM, Rosai J. (1988) “Immunohistochemical detection of epithelial membrane antigen in normal and perineurial cells and perineurioma.” Am J Surg Pathol. 12: 678-83.

小鼠成纤维细胞束膜传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，小鼠成纤维细胞束膜倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
小鼠成纤维细胞束膜其它细胞系展示表：yb-axbz-139 大鼠垂体细胞 RPC 规格25毫升/株.
yb-axbz-140 小鼠垂体细胞 MPC 规格25毫升/株.
yb-axbz-141 兔子垂体细胞 规格25毫升/株.
yb-axbz-142 狗垂体细胞 规格25毫升/株.
yb-axbz-143 鼠肝癌细胞系 MM45T.Li 规格25毫升/株.
yb-axbz-144 小鼠成纤维细胞系 McCoy 规格25毫升/株.
yb-axbz-145 大鼠肝癌细胞（乳腺癌细胞接种到肝脏成瘤） Walker-256 规格25毫升/株.
yb-axbz-146 大鼠肝癌细胞（wistar 大鼠） CBRH7919 规格25毫升/株.
yb-axbz-147 小鼠结肠癌 C26 规格25毫升/株.
yb-axbz-148 稳定转染了neor和LIF基因的STO细胞株 SNL 规格25毫升/株.
yb-axbz-149 小鼠艾氏腹水瘤细胞 ECA 规格25毫升/株.
yb-axbz-150 褐鼠胰岛素瘤上皮细胞 RIN-m 规格25毫升/株.
yb-axbz-151 小鼠杂交瘤细胞 ST2/o 规格25毫升/株.
yb-axbz-152 小鼠肾癌细胞 RenCa 规格25毫升/株.
yb-axbz-153 小鼠胰岛素瘤β细胞（NOD/Lt转基因小鼠，SV40巨T抗原） NIT-1 规格25毫升/株.
yb-axbz-154 早代小鼠胚胎成纤维细胞 MEF 规格25毫升/株.
yb-axbz-155 中国仓鼠肺细胞 V79 规格25毫升/株.
yb-axbz-156 兔的肝细胞 VX2 规格25毫升/株.
yb-axbz-157 鸡胚原代肝细胞 CEL 规格25毫升/株.
yb-axbz-158 129小鼠ES细胞 D3 规格25毫升/株.
yb-axbz-159 129小鼠ES细胞 E14 规格25毫升/株.
yb-axbz-160 BALB/c小鼠ES细胞 B/c-ES 规格25毫升/株.
yb-axbz-161 猴的骨髓间充质干细胞 MSCS 规格25毫升/株.
yb-axbz-162 SD大鼠骨髓间充质干细胞 SDBMSC 规格25毫升/株.
yb-axbz-163 鼠胸腺激酶缺陷细胞株 L-MTK 规格25毫升/株.
yb-axbz-164 鼠胚成纤维细胞系 STO 规格25毫升/株.
yb-axbz-165 鼠乳腺癌细胞系 MA-782 规格25毫升/株.
yb-axbz-166 鼠成纤维细胞系 GR 规格25毫升/株.
yb-axbz-167 杂交瘤细胞枝原体阳性 B6YH4 规格25毫升/株.
yb-axbz-168 鼠细胞系 B82 规格25毫升/株.
编号 中文 英文名称 规格25毫升/株.
yb-axbz-169 兔肾细胞系 RK-13 规格25毫升/株.
yb-axbz-170 猴肾细胞系 MA-104 规格25毫升/株.