产品名称：小鼠脑脊神经元

收到小鼠脑脊神经元时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠脑脊神经元Description

The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain [1]. Despite great variability in size and shape, all neurons share common morphological features, which are those of the key elements of a highly complex communication network. The neurons are the dynamically polarized cells that serve as the major signaling unit of the nervous system.

The MN-r from ScienCell Research Laboratories are isolated from E14 C57BL/6 mouse brain raphe. MN-r are cryopreserved at primary cultures and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. MN-r are characterized by immunofluorescent method with antibodies to neurafilament, MAP2, and beta-tubulin III. MN-r are negative for mycoplasma, bacteria, yeast and fungi. MN-r are guaranteed to further culture in the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Neuronal Medium (NM, Cat. No. 1521) for the culturing of MN-r in vitro.

Product Use

MN-r are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

Reference

[1] Parent, A. (1996) Neurons in Carpenter's Human Neuroanatomy. 9th ed., pp131-198, Williams & Wilkins, Quebec, Canada.

[2] Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, M., Watson, J. D. (1989) Molecular biology of the cell. 2nd. ed., New York: Garland.

小鼠脑脊神经元传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，小鼠脑脊神经元倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
小鼠脑脊神经元其它细胞系展示表：yb-axbz-171 VERO衍生株 SVP 规格25毫升/株.
yb-axbz-172 猪肾细胞系 PK-15 规格25毫升/株.
yb-axbz-173 猪肾细胞系 IBRS-2 规格25毫升/株.
yb-axbz-174 草鱼肾细胞系 GIK 规格25毫升/株.
yb-axbz-175 昆虫细胞系 Sf-21 规格25毫升/株.
yb-axbz-176 昆虫细胞系 SF-9 规格25毫升/株.
yb-axbz-177 草鱼肝脏细胞 L8824 规格25毫升/株.
yb-axbz-178 草鱼肾脏细胞 CIK 规格25毫升/株.
yb-axbz-179 团头鲂尾鳍细胞 WCF 规格25毫升/株.
yb-axbz-180 白鲢尾鳍细胞 SCF 规格25毫升/株.
yb-axbz-181 散鳞镜鲤尾鳍细胞 规格25毫升/株.
yb-axbz-182 鲤鱼尾鳍细胞 规格25毫升/株.
yb-axbz-183 转化的豚鼠胎体细胞 104C1 规格25毫升/株.
yb-axbz-184 中国仓鼠肾细胞(二氢叶酸还原酶缺陷型) CHO/dhFr- 规格25毫升/株.
yb-axbz-185 大鼠乳腺腺癌细胞系 MADB106 规格25毫升/株.
yb-axbz-186 鼠肉瘤细胞 S-180-S2D9 规格25毫升/株.
yb-axbz-187 鼠肝癌细胞 H22-H8D8 规格25毫升/株.
yb-axbz-188 鼠腹水瘤 EAC-E2G8 规格25毫升/株.
yb-axbz-189 小鼠**瘤 EL-4 规格25毫升/株.
yb-axbz-190 鳞癌VX-2(兔模型) 规格25毫升/株.
yb-axbz-191 鲑鱼胚胎细胞 CHSE 规格25毫升/株.
yb-axbz-192 鲑鱼胚胎细胞 RTG 规格25毫升/株.
yb-axbz-193 大黄鱼肾细胞 PCK 规格25毫升/株.
yb-axbz-194 鼠腹水单核细胞瘤 J774A.1 规格25毫升/株.
yb-axbz-195 小鼠IL-3依赖性32D细胞 规格25毫升/株.
yb-axbz-196 大黄鱼EPC细胞 EPC 规格25毫升/株.
yb-axbz-197 菜青虫卵巢细胞 Pr-HNV8 规格25毫升/株.
yb-axbz-198 大鼠胰岛素细胞 INS-1 规格25毫升/株.
yb-axbz-199 小鼠**结血管上皮样细胞 SVEC4-10 规格25毫升/株.
yb-axbz-200 小鼠肉瘤 S37 规格25毫升/株.
yb-axbz-201 小鼠乳腺 EMT6 规格25毫升/株.