产品名称：猪晶状体上皮细胞

收到猪晶状体上皮细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

猪晶状体上皮细胞Description

The cells that make up the mammalian lens consist of two types: lens fiber cells, which form the bulk of the lens, and a monolayer of epithelial cells that cover the anterior surface of the fibers. The normal development of the lens of the eye involves the progressive differentiation and maturation of the lens epithelial cells. As these cells migrate from the equatorial region of the lens into the interior of the lens, they produce the transparent crystallins, elongate to form lens fiber cells, and lose their nuclei and other organelles [1]. The causes of lens epithelial cell differentiation are not well understood; however, some progress has been made in determining the underlying molecular and cellular processes of lens epithelial cell differentiation. This process can be promoted by growth factors present in the ocular fluids [2]. Some growth factors, such as epidermal growth factor, promote mitosis; others, such as basic fibroblast growth factor, insulin growth factor, and insulin, promote cell migration and differentiation [3].

PLEpiC from ScienCell Research Laboratories are isolated from porcine lens. PLEpiC are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. PLEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, cytokeratin-19 and fibronectin. PLEpiC are negative for mycoplasma, bacteria, yeast and fungi. PLEpiC are guaranteed to further expand for 5 population doublings at the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Epithelial Cell Medium (EpiCM, Cat. No. 4101) for the culturing of PLEpiC in vitro.

Product Use

PLEpiC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1]. Wagner, L. M. and Takemoto, D. J. (2001) PKCa and PKC overexpression causes lentoid body formation in the N/N 1003A rabbit lens epithelial cell line. Molecular Vision 7: 138-144.

[2]. Lang, R. A. (1999) Which factors stimulate lens fiber cell differentiation in vivo Invest Opthalmol Vis Sci 40:3075-8.

[3]. Leenders, W. P., van Genesen, S. T., Schoenmakers, J. G., van Zoelen, E. J., Lubsen, N. H. (1997) Synergism between temporally distinct growth factors: bFGF, insulin and lens cell differentiation. Mech Dev. 67:193-201.

猪晶状体上皮细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，猪晶状体上皮细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
猪晶状体上皮细胞其它细胞系展示表：yb-axbz-260 小鼠浆细胞瘤 MPC-11 规格25毫升/株.
yb-axbz-261 转PYTL基因小鼠睾丸支持细胞 15P-1 规格25毫升/株.
yb-axbz-263 小鼠结肠癌细胞 CT26.WT [CT26WT] 规格25毫升/株.
yb-axbz-264 小鼠网织细胞肉瘤 M5076 规格25毫升/株.
yb-axbz-265 小鼠正常肝细胞 NCTC1469 规格25毫升/株.
编号 中文 英文名称 规格25毫升/株.
yb-axbz-266 正常小鼠睾丸Leydig细胞 TM3 规格25毫升/株.
yb-axbz-267 正常小鼠睾丸Leydig细胞 TM4 规格25毫升/株.
yb-axbz-268 615小鼠乳腺癌瘤株 Ca759 规格25毫升/株.
yb-axbz-269 615小鼠乳腺癌瘤株 Ca761 规格25毫升/株.
yb-axbz-270 615小鼠乳腺癌瘤株 Ca763 规格25毫升/株.
yb-axbz-271 615小鼠T细胞性白血病瘤株 L7712 规格25毫升/株.
yb-axbz-272 615小鼠T细胞性白血病瘤株 L7912 规格25毫升/株.
yb-axbz-273 615小鼠肺癌瘤株 HP615 规格25毫升/株.
yb-axbz-274 615小鼠滑膜肉瘤瘤株 ZM755 规格25毫升/株.
yb-axbz-275 615小鼠**状肺腺癌瘤株 P615 规格25毫升/株.
yb-axbz-276 KM小鼠**颈癌瘤株 U27 规格25毫升/株.
yb-axbz-277 KM小鼠网织细胞肉瘤瘤株 LⅡ 规格25毫升/株.
yb-axbz-278 KM小鼠脑神经胶质母细胞瘤瘤株 G422 规格25毫升/株.
yb-axbz-279 615小鼠网织细胞性白血病瘤株 L615 规格25毫升/株.
yb-axbz-280 Walker氏癌肉瘤256瘤株 W256 规格25毫升/株.
yb-axbz-281 C57小鼠黑色素瘤瘤株 ME 规格25毫升/株.
yb-axbz-282 KM小鼠未分化神经胶质瘤瘤株 B22 规格25毫升/株.
yb-axbz-283 小鼠Lewis肺癌瘤株 LLT 规格25毫升/株.
yb-axbz-284 615小鼠前胃癌瘤株 Fc 规格25毫升/株.
yb-axbz-285 小鼠乳腺癌细胞株 EMT-6 规格25毫升/株.
yb-axbz-286 小鼠肾癌细胞株 RuCa 规格25毫升/株.
yb-axbz-287 大鼠肉瘤细胞 WK256 规格25毫升/株.
yb-axbz-288 小鼠乳腺癌细胞 MA891 规格25毫升/株.