产品名称：猪视网膜色素上皮细胞

收到猪视网膜色素上皮细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

猪视网膜色素上皮细胞Description

The retina is a multi-layered structure lining the rear of the eye. It consists of photoreceptor cells and retinal pigment epithelial cells (RPE). RPE situated between the neurosensory retina and the choroid, form the outer blood-retina barrier that controls the chemical composition of the subretinal space [1]. RPE has long been recognized to differ from many other epithelia in the polarity of a number of proteins and whereas almost all other epithelia face an apical lumen devoid of matrix, the RPE apical plasma membrane is in contact with the extracellular matrix [2]. During early embryonic development, RPE spontaneously transdifferentiate into neural retinal tissue [3]. RPE play a critical role in the development and maintenance of adjacent photoreceptors. They generate a number of reactive oxygen species when illuminated with light and their proliferation is an important step in the pathogenesis of ocular diseases such as proliferative retinopathy.

PRPEpiC from ScienCell Research Laboratories are isolated from porcine retina. PRPEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. PRPEpiC are characterized by immunofluorescent method with antibodies to cytokeratin-18, cytokeratin-19 and fibronectin. PRPEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. PRPEpiC are guaranteed to further expand for 15 population doublings in the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Epithelial Cell Medium (EpiCM, Cat No. 1401) for expanding PRPEpiC in vitro.

Product Use

PRPEpC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1] Rizzolo, L. J. (1997) Polarity and the development of the outer blood-retinal barrier. Histol Histopathol 12:1057-1067.

[2] Marmorstein, A. D. (2001) The polarity of the retinal pigment epithelium. Traffic 2:867-872.

[3] Araki, M., Takano, T., Uemonsa, T., Nakane, Y., Tsudzuki, M., Kaneko, T. (2002) Epithelia-mesenchyme interaction plays an essential role in transdifferentiation of retinal pigment epithelium of silver mutant quail: localization of FGF and related molecules and aberrant migration pattern of neural crest cells during eye rudiment formation. Dev Biol. 244:358-71.

猪视网膜色素上皮细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，猪视网膜色素上皮细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
猪视网膜色素上皮细胞其它细胞系展示表：yb-axbz-289 大鼠胰腺癌细胞株 DSL-6A/C1 规格25毫升/株.
yb-axbz-290 小鼠骨髓瘤细胞 NS1 规格25毫升/株.
yb-axbz-291 大鼠卵巢癌细胞株 NUTU-19 规格25毫升/株.
yb-axbz-292 小鼠肾癌细胞 Ranca 规格25毫升/株.
yb-axbz-293 小鼠胚胎成纤维细胞 3T3-swiss albino 规格25毫升/株.
yb-axbz-294 小鼠胚胎成纤维细胞 3T3 clone A31 规格25毫升/株.
yb-axbz-295 小鼠胰腺腺泡细胞癌细胞 MPC-83 规格25毫升/株.
编号 中文 英文名称 规格25毫升/株.
yb-axbz-296 小鼠白血病细胞 C3 规格25毫升/株.
yb-axbz-297 小鼠β胰岛素瘤细胞 RIN-m5F 规格25毫升/株.
yb-axbz-298 小鼠自发高乳腺癌细胞 TA2 规格25毫升/株.
yb-axbz-299 小鼠**瘤细胞 Nb2－11 规格25毫升/株.
yb-axbz-300 小鼠骨髓瘤细胞 NS-1 规格25毫升/株.
yb-axbz-301 小鼠肾癌细胞 Kert-3 规格25毫升/株.
yb-axbz-302 HPVl6 E6、E7和ras基因共转化的C57BL／C(H-2b)小鼠肺上皮细胞 TC-1 规格25毫升/株.
yb-axbz-303 长臂猿**瘤 MLA-144 规格25毫升/株.
货号. 名称缩写 中文名称 英文名称 规格
Nervous System 神经系统 品牌
yb1000 HBMEC 人脑微血管内皮细胞 Human Brain Microvascular Endothelial Cells 5 x 10^5 cells/vial ATCC
yb1001 HBVSMC 人脑血管平滑肌细胞 Human Brain Vascular Smooth Muscle Cells 5 x 10^5 cells/vial ATCC
yb1002 HBVAF 人脑血管外膜成纤维细胞 Human Brain Vascular Adventitial Fibroblasts 5 x 10^5 cells/vial ATCC
yb1003 HBVP 人脑血管周细胞 Human Brain Vascular Pericytes 5 x 10^5 cells/vial ATCC
yb1004 HCPEC 人脉络丛内皮细胞 Human Choroid Plexus Endothelial Cells 5 x 10^5 cells/vial ATCC
yb1005 HCPEpiC 人脉络丛上皮细胞 Human Choroid Plexus Epithelial Cells 5 x 10^5 cells/vial ATCC
yb1006 HCPF 人脉络丛成纤维细胞 Human Choroid Plexus Fibroblasts 5 x 10^5 cells/vial ATCC
yb1007 HMC 人脑膜细胞 Human Meningeal Cells 5 x 10^5 cells/vial ATCC
yb1008 HN 人神经元细胞 Human Neurons 1 x 10^6 cells/vial ATCC