产品名称：膜铁转运蛋白(FPN)单克隆抗体

膜铁转运蛋白(FPN)单克隆抗体

• 来源单抗制备
• 宿主小鼠
• 效价(克隆号)C7
• Ig亚型IgG2b Kappa
• 纯化方式蛋白A + 蛋白G亲和纯化
• 标记物无标记物
• **原RPC489Hu01-膜铁转运蛋白(FPN)重组蛋白
• 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
• 性状液体
• 浓度500ug/ml
• 且适物种-
• 应用WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM

特异性
该抗体是针对FPN的小鼠单克隆抗体。在**组织化学染色和**印迹实验中能识别FPN。
用法
Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过一年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
YBC489Hu21

膜铁转运蛋白(FPN)单克隆抗体Monoclonal Antibody to Ferroportin (FPN)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

11th Edition (Revised in May, 2016)

[ PROPERTIES ]

Source: Monoclonal antibody preparation

Host: Mouse

Antibody isotype: IgG2b Kappa

Purification: Protein A/G Affinity Chromatography. Clone number: C7

Traits: Liquid

Concentration: 500µg/mL

UOM: 200µg

Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]

Immunogen: RPC489Hu01-Recombinant Ferroportin (FPN)

膜铁转运蛋白(FPN)单克隆抗体[ APPLICATIONS ]

Western blotting: 0.5-5ug/ml

Immunocytochemistry in formalin fixed cells: 5-30ug/ml

Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml

Immunohistochemistry in paraffin section: 5-30ug/ml

Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml

Optimal working dilutions must be determined by end user. [ FORMULATION ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant FPN disposed in loading

buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine (DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 4

oC for frequent use. Aliquot and store at -20

oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate

was determined by accelerated thermal degradation test, that is, incubate the

protein at 37

oC for 48h, and no obvious degradation and precipitation were

observed.The loss rate is less than 5% within the expiration date under

appropriate storage condition.膜铁转运蛋白(FPN)单克隆抗体 [ IDENTIFICATION ]

A

Figure 1. Western Blot

A. Sample: Recombinant FPN, Human B. Rat Placenta Tissue Primary Ab: 2µg/mL Rabbit Anti-Human FPN Ab

Second Ab: 1:5000 Dilution of HRP-Linked Rabbit

Anti-Mouse IgG Ab (Catalog: SAA544Mu09)

B

膜铁转运蛋白(FPN)单克隆抗体Figure 2. DAB staining on IHC-P Samples： A. Human Brain Tissue B. Human Glioma Tissue C. Human Stomach Tissue D. Human Rectum Cancer Tissue A B C D

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