- 入驻时间： 2015-11-06
- 联系人：顾磊
- 电话：021-60514606
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联系时，请说明易展网看到的
- Email：shybio@126.com
- 衰老关键蛋白1(FBLN1)单克隆抗体来源单抗制备
- 宿主小鼠
- 效价(克隆号)C6
- Ig亚型IgG2a Kappa
- 纯化方式蛋白A + 蛋白G亲和纯化
- 标记物无标记物
- **原RPC472Hu01-衰老关键蛋白1(FBLN1)重组蛋白
- 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
- 性状液体
- 浓度500ug/ml
- 且适物种-
- 应用WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM
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衰老关键蛋白1(FBLN1)单克隆抗体特异性
该抗体是针对FBLN1的小鼠单克隆抗体。在**组织化学染色和**印迹实验中能识别FBLN1。
用法
Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过一年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
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YBC472Hu21
衰老关键蛋白1(FBLN1)单克隆抗体Monoclonal Antibody to Fibulin 1 (FBLN1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Monoclonal antibody preparation
Host: Mouse
Antibody isotype: IgG2a Kappa
Purification: Protein A/G Affinity Chromatography. Clone number: C6
Traits: Liquid
Concentration: 500µg/mL
UOM: 200µg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]
Immunogen: RPC472Hu01-Recombinant Fibulin 1 (FBLN1)
[ APPLICATIONS ]Western blotting: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user. [ FORMULATION ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
衰老关键蛋白1(FBLN1)单克隆抗体[ QUALITY CONTROL ]
Content: The quality control contains recombinant FBLN1 disposed in loading
buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine (DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 4
oC for frequent use. Aliquot and store at -20
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ IDENTIFICATION ]
A
衰老关键蛋白1(FBLN1)单克隆抗体Figure 1. Western Blot
A. Sample: Recombinant FBLN1, Human B. Lane1: Human Serum
Lane2: Rat Placenta Tissue Primary Ab: 2µg/mL Rabbit Anti-Human FBLN1 Ab
Second Ab: 1:5000 Dilution of HRP-Linked Rabbit
Anti-Mouse IgG Ab (Catalog: SAA544Mu09)
B
