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- 联系人:顾磊
- 电话:021-60514606
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- Email:shybio@126.com
白细胞激活黏附因子(ALCAM)单克隆抗体
- 来源单抗制备
- 宿主小鼠
- 效价(克隆号)-
- Ig亚型IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- **原-
- 缓冲液成份磷酸盐缓冲液(pH7.4,含有0.02% NaN3和50%甘油)
- 性状液体
- 浓度500ug/ml
- 且适物种-
-
应用WB, ICC, IHC-P, IHC-F, ELISA
白细胞激活黏附因子(ALCAM)单克隆抗体特异性
该抗体是针对ALCAM的小鼠单克隆抗体。在**组织化学染色和**印迹实验中能识别ALCAM。
用法
**印迹:1:100-400
**细胞化学:1:50-500
**组织化学(冰冻切片):1:50-500
**组织化学(石蜡切片):1:10-100
酶联**吸附试验:1:100-1:5000
*佳稀释倍数*终由用户决定。
储存
经常使用则4°C保存。-20°C保存不超过一年。避免反复冻融。
白细胞激活黏附因子(ALCAM)单克隆抗体稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。白细胞激活黏附因子(ALCAM)单克隆抗体
Monoclonal Antibody to Activated Leukocyte Cell Adhesion Molecule (ALCAM)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: ALCAM, Human
Clonality: Monoclonal
Clone number: A5
Host: Mouse
Immunoglobulin Type: IgG
Purification: 白细胞激活黏附因子(ALCAM)单克隆抗体Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 500µg/mL
UOM: 200µg Sample: Recombinant ALCAM, Human
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant ALCAM (Trp28~Lys527) expressed in E.coli. Accession No.: RPA002Hu01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence
is listed below.
白细胞激活黏附因子(ALCAM)单克隆抗体MGHHHHHHSGSEF-WYT VNSAYGDTII IPCRLDVPQN LMFGKWKYEK PDGSPVFIAF
RSSTKKSVQY DDVPEYKDRL NLSENYTLSI SNARISDEKR FVCMLVTEDN VFEAPTIVKV
FKQPSKPEIV SKALFLETEQ LKKLGDCISE DSYPDGNITW YRNGKVLHPL EGAVVIIFKK
EMDPVTQLYT MTSTLEYKTT KADIQMPFTC SVTYYGPSGQ KTIHSEQAVF DIYYPTEQVT
IQVLPPKNAI KEGDNITLKC LGNGNPPPEE FLFYLPGQPE GIRSSNTYTL TDVRRNATGD
YKCSLIDKKS MIASTAITVH YLDLSLNPSG EVTRQIGDAL PVSCTISASR NATVVWMKDNIRLRSSPSFS SLHYQDAGNY VCETALQEVE GLKKRESLTL IVEGKPQIKM TKKTDPSGLS
KTIICHVEGF PKPAIQWTIT GSGSVINQTE ESPYINGRYY SKIIISPEEN VTLTCTAENQ
LERTVNSLNV SAISIPEHDE ADEISDENRE KVNDQAK
[ ANTIBODY SPECIFITY ]
The antibody is a mouse monoclonal antibody raised against ALCAM. It has been
selected for its ability to recognize ALCAM in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
白细胞激活黏附因子(ALCAM)单克隆抗体Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.