- 入驻时间: 2015-11-06
- 联系人:顾磊
- 电话:021-60514606
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- Email:shybio@126.com
血管生成素1(ANGPT1)单克隆抗体
- 来源单抗制备
- 宿主小鼠
- 效价(克隆号)C5
- Ig亚型IgG
- 纯化方式蛋白A + 蛋白G亲和纯化
- 标记物无标记物
- **原RPA008Hu01-血管生成素1(ANGPT1)重组蛋白
- 缓冲液成份磷酸盐缓冲液(pH7.4,含有0.02% NaN3和50%甘油)
- 性状液体
- 浓度500ug/ml
- 且适物种-
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应用WB, ICC, IHC-P, IHC-F, ELISA
血管生成素1(ANGPT1)单克隆抗体特异性
该抗体是针对ANGPT1的小鼠单克隆抗体。在**组织化学染色和**印迹实验中能识别ANGPT1。
用法
**印迹:1:100-400
**细胞化学:1:50-500
**组织化学(冰冻切片):1:50-500
**组织化学(石蜡切片):1:10-100
酶联**吸附试验:1:100-1:5000
*佳稀释倍数*终由用户决定。
储存
经常使用则4°C保存。-20°C保存不超过一年。避免反复冻融。
血管生成素1(ANGPT1)单克隆抗体稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。Monoclonal Antibody to Angiopoietin 1 (ANGPT1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: ANGPT1, Human
Clonality: Monoclonal
Clone number: C5
Host: Mouse血管生成素1(ANGPT1)单克隆抗体
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 500µg/mL
UOM: 200µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant ANGPT1 (Arg284~Cys452) with N-terminal His-Tag
expressed in E.coli. Accession No.: RPA008Hu01
[ ANTIBODY SPECIFITY ]
The antibody is a mouse monoclonal antibody raised against ANGPT1. It has been
血管生成素1(ANGPT1)单克隆抗体selected for its ability to recognize ANGPT1 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Optimal working dilutions must be determined by end user.[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant ANGPT1 (Arg284~Cys452)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4血管生成素1(ANGPT1)单克隆抗体
oC for frequent use. Stored at -20oC in a manual defrost freezer for one
year without detectable loss of activity. Avoid repeated freeze-thaw cycles.