产品名称：谷丙转氨酶(ALT)单克隆抗体

谷丙转氨酶(ALT)单克隆抗体

• 来源单抗制备
• 宿主小鼠
• 效价(克隆号)11#
• Ig亚型IgG2b Kappa
• 纯化方式蛋白A + 蛋白G亲和纯化
• 标记物无标记物
• **原RPA207Hu03-谷丙转氨酶(ALT)重组蛋白
• 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
• 性状液体
• 浓度500ug/ml
• 且适物种-
• 应用WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM

  谷丙转氨酶(ALT)单克隆抗体特异性

  该抗体是针对ALT的小鼠单克隆抗体。在**组织化学染色和**印迹实验中能识别ALT。

  用法

  Western blotting: 0.5-5ug/ml

  Immunocytochemistry in formalin fixed cells: 5-30ug/ml

  Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml

  Immunohistochemistry in paraffin section: 5-30ug/ml

  Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml

  Optimal working dilutions must be determined by end user.

  储存

  经常使用则4°C保存。-20°C保存不超过一年。避免反复冻融。

  谷丙转氨酶(ALT)单克隆抗体稳定性

  热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。MAA207Hu22
  Monoclonal Antibody to Alanine Aminotransferase (ALT)
  Organism Species: Homo sapiens (Human)
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  11th Edition (Revised in May, 2016)
  [ PROPERTIES ]谷丙转氨酶(ALT)单克隆抗体
  Source: Monoclonal antibody preparation
  Host: Mouse
  Antibody isotype: IgG2b Kappa
  Purification: Protein A/G Affinity Chromatography. Clone number: 11#
  Traits: Liquid
  Concentration: 500µg/mL
  UOM: 200µg谷丙转氨酶(ALT)单克隆抗体
  Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]
  Immunogen: RPA207Hu03-Recombinant Alanine Aminotransferase (ALT)
  [ APPLICATIONS ]
  Western blotting: 0.5-5ug/ml
  Immunocytochemistry in formalin fixed cells: 5-30ug/ml
  Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
  Immunohistochemistry in paraffin section: 5-30ug/ml
  Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
  Optimal working dilutions must be determined by end user. [ FORMULATION ]
  Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.[ QUALITY CONTROL ]
  Content: The quality control contains recombinant ALT disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine (DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ]
  Storage: Avoid repeated freeze/thaw cycles. Store at 4
  oC for frequent use. Aliquot and store at -20
  oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
  was determined by accelerated thermal degradation test, that is, incubate the
  protein at 37
  oC for 48h, and no obvious degradation and precipitation were
  observed.The loss rate is less than 5% within the expiration date under
  appropriate storage condition. [ IDENTIFICATION ]
  A
  Figure 1. Western Blot
  A. Sample: Recombinant ALT, HumanFigure 2. DAB staining on
  IHC-P
  Samples：
  A. Human Glioma Tissue B. Human Liver Cancer Tissue