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第7年
- 入驻时间： 2015-11-06
- 联系人：顾磊
- 电话：021-60514606
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联系时，请说明易展网看到的
- Email：shybio@126.com
产品分类
产品详情
简单介绍：
188kDa核孔蛋白(NUP188)重组蛋白蛋白质的不同在于其氨基酸的种类、数目、排列顺序和肽链空间结构的不同，蛋白质的氨基酸序列由对应基因所编码，空间结构决定于氨基酸序列本身，通过氢键或范德华力等作用力实现。蛋白质不仅具有**结构，还具有二级、三级结构，甚至四级结构。**结构只是蛋白质的基本结构，称之为低级结构。188kDa核孔蛋白(NUP188)重组蛋白如果想让蛋白质有更多的生物功能则必须赋予它们更复杂的空间结构，称之为**结构。应用重组DNA将目标基因进行表达得到的
详情介绍：
188kDa核孔蛋白(NUP188)重组蛋白
- 物种Homo sapiens (Human，人)
- E.coli
- 内**水平<1.0EU/µg(LAL法测定)
- 亚细胞定位n/a
- 预测分子量53.8kDa
- 实际分子量-(差异分析请参阅说明书)
- 片段与标签Thr540~Gly782 (Accession # Q5SRE5) with N-terminal His Tag
- 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性状冻干粉
- 纯度> 95%
- 等电点-
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应用SDS-PAGE; WB; ELISA; IP
188kDa核孔蛋白(NUP188)重组蛋白用法
PBS或其它。
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
188kDa核孔蛋白(NUP188)重组蛋白
Nucleoporin 188kDa (NUP188)
Organism: Homo sapiens (Human)
Instruction manual188kDa核孔蛋白(NUP188)重组蛋白
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
5th Edition (Revised in January, 2013)
[ DESCRIPTION ]
Protein Names: Nucleoporin 188kDa
Synonyms: NUP188, KIAA0169
Species: Human
Size: 100µg
Source: Escherichia coli-derived
Subcellular Location: Nucleus, nuclear pore
complex.
[ PROPERTIES ]
Residues: Thr540~Gly782 (Accession # Q5SRE5),
with two N-terminal Tags, His-tag and GST-tag. Grade & Purity: >95%, 55kDa as determined by
SDS-PAGE reducing conditions.
Formulation: Supplied as lyophilized form in PBS, pH
7.4, containing 5% sucrose, 0.01% sarcosyl. Endotoxin Level: <1.0 EU per 1μg (determined by
the LAL method).
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Molecular Mass: 53.8kDa188kDa核孔蛋白(NUP188)重组蛋白
Predicted isoelectric point: 6.4
Human NUP188[ PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL
DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD
VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD
GS T S GS G H H H HH HSAG LVP R GST AIG MKET AAAK F E R Q HM DSP D LG T LEV
LFQGPLGSEF- T LFTCEIEMLL HVVSTADVIQ HCQRVKPIID LVHKVISTDL SIADCLLPIT
SRIYMLLQRL TTVISPPVDV IASCVNCLTV LAARNPAKVW TDLRHTGFLP FVAHPVSSLS
QMISAEGMNA GGYGNLLMNS EQPQGEYGVT IAFLRLITTL VKGQLGSTQS QGLVPCVMFV
LKEMLPSYHK WRYNSHGVRE QIGCLILELI HAILNLCHET DLHSSHTPSL QFLCICSLAY
TEAGQTVINI MG
