企业信息
第7年
- 入驻时间： 2015-11-06
- 联系人：顾磊
- 电话：021-60514606
-
联系时，请说明易展网看到的
- Email：shybio@126.com
产品分类
产品详情
简单介绍：
25kDa突触关联蛋白(SNAP25)重组蛋白蛋白质的不同在于其氨基酸的种类、数目、排列顺序和肽链空间结构的不同，蛋白质的氨基酸序列由对应基因所编码，空间结构决定于氨基酸序列本身，通过氢键或范德华力等作用力实现。蛋白质不仅具有**结构，还具有二级、三级结构，甚至四级结构。**结构只是蛋白质的基本结构，称之为低级结构。25kDa突触关联蛋白(SNAP25)重组蛋白如果想让蛋白质有更多的生物功能则必须赋予它们更复杂的空间结构，称之为**结构。应用重组DNA将目标基因进行表达得到的
详情介绍：
25kDa突触关联蛋白(SNAP25)重组蛋白
- 物种Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- E.coli
- 内**水平<1.0EU/µg(LAL法测定)
- 亚细胞定位n/a
- 预测分子量27.0kDa
- 实际分子量27kDa(差异分析请参阅说明书)
- 片段与标签Met1~Gly206 with N-terminal His Tag
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 97%
- 等电点4.7
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应用SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling
25kDa突触关联蛋白(SNAP25)重组蛋白用法
PBS或其它。
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
25kDa突触关联蛋白(SNAP25)重组蛋白
Recombinant Synaptosomal Associated Protein 25kDa (SNAP25)
Organism Species: Multi-species
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Met1~Gly206
Tags: N-terminal His-Tag25kDa突触关联蛋白(SNAP25)重组蛋白
Purity: >98%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive
Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 4.7
Predicted Molecular Mass: 27.0kDa
Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions. Note: Full length human SNAP25 is identical in sequence to mouse and rat. 100%25kDa突触关联蛋白(SNAP25)重组蛋白
cross-reactivity of SNAP25 was observed among human, mouse and rat. [ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0
mg/mL. Do not vortex.[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. SDS-PAGE
