产品名称：Ⅱ型胶原α1(COL2a1)重组蛋白

Ⅱ型胶原α1(COL2a1)重组蛋白

• 物种Homo sapiens (Human，人)
• E.coli
• 内**水平<1.0EU/µg(LAL法测定)
• 亚细胞定位n/a
• 预测分子量10.6kDa
• 实际分子量-(差异分析请参阅说明书)
• 片段与标签Ser1386~Pro1467 (Accession # P02458) with N-terminal His Tag
• 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
• 性状冻干粉
• 纯度> 95%
• 等电点-
• 应用SDS-PAGE; WB; ELISA; IP

  
  

  Ⅱ型胶原α1(COL2a1)重组蛋白用法

  PBS或其它。

  储存

  避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

  稳定性

  热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。Ⅱ型胶原α1(COL2a1)重组蛋白
  Collagen Type II Alpha 1 (COL2a1)
  Organism: Homo sapiens (Human)
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  5th Edition (Revised in January, 2013)
  [ DESCRIPTION ]
  Protein Names: Collagen Type II Alpha 1
  Synonyms: COL2a1
  Species: Human
  Size: 100µgⅡ型胶原α1(COL2a1)重组蛋白
  Source: Escherichia coli-derived
  Subcellular Location: Secreted, extracellular space,
  extracellular matrix.
  [ PROPERTIES ]
  Residues: Ser1386~Pro1467 (Accession # P02458),
  with N-terminal His-Tag. Grade & Purity: >95%, 12kDa as determined by
  SDS-PAGE reducing conditions.
  Formulation: Supplied as lyophilized form in PBS, pH
  7.4, containing 5% sucrose, 0.01% sarcosyl. Endotoxin Level: <1.0 EU per 1μg (determined by
  the LAL method).
  Applications: SDS-PAGE; WB; ELISA; IP.
  (May be suitable for use in other assays to be determined by the end user.)
  Predicted Molecular Mass: 10.6kDa
  Predicted isoelectric point: 9.0
  Human COL2a1[ PREPARATION ]
  Reconstitute in sterile PBS, pH7.2-pH7.4.
  [ STORAGE AND STABILITY ]
  Storage: Avoid repeated freeze/thaw cyclesⅡ型胶原α1(COL2a1)重组蛋白.
  Store at 2-8oC for one month.
  Aliquot and store at -80oC for 12 months.
  Stability Test: The thermal stability is described by the loss rate of the target
  protein. The loss rate was determined by accelerated thermal degradation test,
  that is, incubate the protein at 37oC for 48h, and no obvious degradation and
  precipitation were observed. (Referring from China Biological Products Standard,
  which was calculated by the Arrhenius equation.) The loss of this protein is less
  than 5% within the expiration date under appropriate storage condition.
  [ SEQUENCES ]
  The target protein is fused with N-terminal His-Tag, its sequence is listed below.
  MGHHHHHHSGSEF- SQNIT YHCKNSIAYL DEAAGNLKKA LLIQGSNDVE IRAEGNSRFT
  YTALKDGCTK HTGKWGKTVI EYRSQKTSRL PIIDIAP