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第7年
- 入驻时间： 2015-11-06
- 联系人：顾磊
- 电话：021-60514606
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联系时，请说明易展网看到的
- Email：shybio@126.com
产品分类
产品详情
简单介绍：
Ⅷ型胶原α1(COL8a1)重组蛋白蛋白质的不同在于其氨基酸的种类、数目、排列顺序和肽链空间结构的不同，蛋白质的氨基酸序列由对应基因所编码，空间结构决定于氨基酸序列本身，通过氢键或范德华力等作用力实现。蛋白质不仅具有**结构，还具有二级、三级结构，甚至四级结构。**结构只是蛋白质的基本结构，称之为低级结构。Ⅷ型胶原α1(COL8a1)重组蛋白如果想让蛋白质有更多的生物功能则必须赋予它们更复杂的空间结构，称之为**结构。应用重组DNA将目标基因进行表达得到的
详情介绍：
Ⅷ型胶原α1(COL8a1)重组蛋白
- 物种Homo sapiens (Human，人)
- E.coli
- 内**水平<1.0EU/µg(LAL法测定)
- 亚细胞定位Secreted, extracellular
- 预测分子量20.2kDa
- 实际分子量-(差异分析请参阅说明书)
- 片段与标签Pro575~Pro743 (Accession # P27658) with N-terminal His Tag
- 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性状冻干粉
- 纯度> 95%
- 等电点7.1
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应用SDS-PAGE; WB; ELISA; IP
Ⅷ型胶原α1(COL8a1)重组蛋白用法
PBS或其它。
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
Ⅷ型胶原α1(COL8a1)重组蛋白
Recombinant Collagen Type VIII Alpha 1 (COL8a1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]Ⅷ型胶原α1(COL8a1)重组蛋白
Residues: Pro575~Pro743 (Accession # P27658),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Secreted, extracellular
space, extracellular matrix, basement membrane
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 7.1
Predicted Molecular Mass: 20.2kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.Ⅷ型胶原α1(COL8a1)重组蛋白
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-PPTPPP QGEYLPDMGL GIDGVKPPHA YGAKKGKNGG PAYEMPAFTA
ELTAPFPPVG APVKFNKLLY NGRQNYNPQT GIFTCEVPGV YYFAYHVHCK GGNVWVALFK
NNEPVMYTYD EYKKGFLDQA SGSAVLLLRP GDRVFLQMPSEQAAGLYAGQ YVHSSFSGYL
LYP
[ REFERENCES ]
1. Muragaki Y., et al. (1991) Eur. J. Biochem. 197:615-622. 2. Kittelberger R., et al. (1990) Connect. Tissue Res. 24:303-318. 3. Kittelberger R., et al. (1992) Biochim. Biophys. Acta 1139:295-299. 4. Ruger B., et al. (1994) Int. J. Exp. Pathol. 75:397-404.
