企业信息
第7年
- 入驻时间： 2015-11-06
- 联系人：顾磊
- 电话：021-60514606
-
联系时，请说明易展网看到的
- Email：shybio@126.com
产品分类
产品详情
简单介绍：
90kDa热休克蛋白αA1(HSP90aA1)重组蛋白蛋白质的不同在于其氨基酸的种类、数目、排列顺序和肽链空间结构的不同，蛋白质的氨基酸序列由对应基因所编码，空间结构决定于氨基酸序列本身，通过氢键或范德华力等作用力实现。蛋白质不仅具有**结构，还具有二级、三级结构，甚至四级结构。**结构只是蛋白质的基本结构，称之为低级结构。90kDa热休克蛋白αA1(HSP90aA1)重组蛋白如果想让蛋白质有更多的生物功能则必须赋予它们更复杂的空间结构，称之为**结构。应用重组DNA将目标基因进行表达得到的
详情介绍：
90kDa热休克蛋白αA1(HSP90aA1)重组蛋白
- 物种Homo sapiens (Human，人)
- E.coli
- 内**水平<1.0EU/µg(LAL法测定)
- 亚细胞定位n/a
- 预测分子量85.9kDa
- 实际分子量-(差异分析请参阅说明书)
- 片段与标签Met1~Asp732 with N-terminal His Tag
- 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性状冻干粉
- 纯度> 95%
- 等电点-
-
应用SDS-PAGE; WB; ELISA; IP
90kDa热休克蛋白αA1(HSP90aA1)重组蛋白用法
PBS或其它。
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
90kDa热休克蛋白αA1(HSP90aA1)重组蛋白
Recombinant Heat Shock Protein 90kDa Alpha A1 (HSP90aA1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PROPERTIES ]
Residues: Met1~Asp732
Tags: N-terminal His-Tag
Accession: P0790090kDa热休克蛋白αA1(HSP90aA1)重组蛋白
Host: E. coli
Subcellular Location: Cytoplasm. Melanosome.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.0
Predicted Molecular Mass: 85.9kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: 99.9% cross-reactivity of HSP90aA1 was observed among human, mouse
and rat.
[ USAGE ]
Reconstitute in ddH2O.[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.90kDa热休克蛋白αA1(HSP90aA1)重组蛋白
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MPEETQTQDQ PMEEEEVETF AFQAEIAQLM SLIINTFYSN KEIFLRELIS NSSDALDKIR
YESLTDPSKL DSGKELHINL IPNKQDRTLT IVDTGIGMTK ADLINNLGTI AKSGTKAFME
ALQAGADISM IGQFGVGFYS AYLVAEKVTV ITKHNDDEQY AWESSAGGSF TVRTDTGEPM
GRGTKVILHL KEDQTEYLEE RRIKEIVKKH SQFIGYPITL FVEKERDKEV SDDEAEEKED
KEEEKEKEEK ESEDKPEIED VGSDEEEEKK DGDKKKKKKI KEKYIDQEEL NKTKPIWTRN
PDDITNEEYG EFYKSLTNDW EDHLAVKHFS VEGQLEFRAL LFVPRRAPFD LFENRKKKNN
IKLYVRRVFI MDNCEELIPE YLNFIRGVVD SEDLPLNISR EMLQQSKILK VIRKNLVKKC
LELFTELAED KENYKKFYEQ FSKNIKLGIH EDSQNRKKLS ELLRYYTSAS GDEMVSLKDY
CTRMKENQKH IYYITGETKD QVANSAFVER LRKHGLEVIY MIEPIDEYCV QQLKEFEGKT
LVSVTKEGLE LPEDEEEKKK QEEKKTKFEN LCKIMKDILE KKVEKVVVSN RLVTSPCCIV
TSTYGWTANM ERIMKAQALR DNSTMGYMAA KKHLEINPDH SIIETLRQKA EADKNDKSVK
DLVILLYETA LLSSGFSLED PQTHANRIYR MIKLGLGIDE DDPTADDTSA AVTEEMPPLE
GDDDTSRMEE VD
[ REFERENCES ]
1. Soeda E., et al. (1989) Nucleic Acids Res. 17:7108-7108. 2. Yamazaki M., et al. (1990) Agric. Biol. Chem. 54:3163-3170. 3. Chen B., et al. (2005) Genomics 86:627-637. 4. Hoffmann T., Hovemann B. (1988) Gene 74:491-501.
