产品名称：粘蛋白17(MUC17)重组蛋白

粘蛋白17(MUC17)重组蛋白

• 物种Homo sapiens (Human，人)相同的名称，不同的物种。
• 片段与标签Thr3773~Thr3998 with N-terminal His Tag宿主E.coli
• 内**水平<1.0EU/µg(LAL法测定)
• 亚细胞定位n/a
• 预测分子量24.3kDa
• 实际分子量-(差异分析请参阅说明书)
• 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
• 性状冻干粉
• 纯度> 95%
• 等电点-
• 应用SDS-PAGE; WB; ELISA; IP
  粘蛋白17(MUC17)重组蛋白用法
  PBS或其它。
  储存
  避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
  稳定性
  热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
  
  粘蛋白17(MUC17)重组蛋白

  
  

  Recombinant Mucin 17 (MUC17)

  Organism Species: Homo sapiens (Human)

  Instruction manual

  FOR IN VITRO USE AND RESEARCH USE ONLY

  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

  10th Edition (Revised in Jan, 2014)

  粘蛋白17(MUC17)重组蛋白[ PROPERTIES ]

  Residues: Thr3773~Thr3998

  Tags: N-terminal His-Tag

  Accession: Q685J3

  Host: E. coli

  Subcellular Location: Cell membrane;

  Single-pass membrane protein.

  Purity: >95%

  Endotoxin Level: <1.0EU per 1μg

  (determined by the LAL method).

  Formulation: Supplied as lyophilized form in 10mM

  PBS, pH7.4, containing 1mM DTT, 5% trehalose,

  0.01% sarcosyl and preservative.

  Predicted isoelectric point: 4.9

  Predicted Molecular Mass: 24.3kDa

  Accurate Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions.

  Applications: SDS-PAGE; WB; ELISA; IP.

  (May be suitable for use in other assays to be determined by the end user.)

  Note: The possible reasons that the actual band size differs from the predicted are as follows:

  1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

  2. Relative charge: The composition of amino acids may affects the charge of the protein.

  3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

  4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

  give the active form.

  5. Polymerization of the target protein: Dimerization, multimerization etc.

  粘蛋白17(MUC17)重组蛋白[ USAGE ]

  Reconstitute in sterile ddH2O.

  [ STORAGE AND STABILITY ]

  Storage: Avoid repeated freeze/thaw cycles.

  Store at 2-8

  oC for one month.

  Aliquot and store at -80oC for 12 months.

  Stability Test: The thermal stability is described by the loss rate of the target

  protein. The loss rate was determined by accelerated thermal degradation test,

  that is, incubate the protein at 37oC for 48h, and no obvious degradation and

  precipitation were observed. (Referring from China Biological Products Standard,

  which was calculated by the Arrhenius equation.) The loss of this protein is less

  than 5% within the expiration date under appropriate storage condition.

  [ SEQUENCES ]

  The sequence of the target protein is listed below.

  TPSIPSVY TSMSMTTASE GSSSPTTLEG TTTMPMSTTS ERSTLLTTVL ISPISVMSPS

  EASTLSTPPG DTSTPLLTST KAGSFSIPAE VTTIRISITS ERSTPLTTLL VSTTLPTSFP

  GASIASTPPL DTSTTFTPST DTASTPTIPV ATTISVSVIT EGSTPGTTIF IPSTPVTSST

  ADVFPATTGA VSTPVITSTE LNTPSTSSSS TTTSFSTTKE FTTPAMTT

  [ REFERENCES ]

  1. Malmberg E.K., et al. (2007) Biochem. J. 410:283-289.

  2. Ho J.J.L., et al. (2002) Int. J. Oncol. 23:585-592.

  3. Gum J.R. Jr., et al. (2001) Biochem. Biophys. Res. Commun. 291:466-475.

  4. Van Klinken B.J.-W., et al. (1996) Biochem. Biophys. Res. Commun. 238:143-148.