产品名称：轴突生长诱向因子1(Ntn1)重组蛋白

轴突生长诱向因子1(Ntn1)重组蛋白

• 物种Homo sapiens (Human，人)相同的名称，不同的物种。
• 片段与标签Pro313~Pro565 (Accession # O95631) with N-terminal His Tag宿主E.coli
• 内**水平<1.0EU/µg(LAL法测定)
• 亚细胞定位n/a
• 预测分子量55.5kDa
• 实际分子量-(差异分析请参阅说明书)
• 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
• 性状冻干粉
• 纯度> 95%
• 等电点-
• 应用SDS-PAGE; WB; ELISA; IP
  轴突生长诱向因子1(Ntn1)重组蛋白用法
  PBS或其它。
  储存
  避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
  稳定性
  热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
  
  轴突生长诱向因子1(Ntn1)重组蛋白

  
  

  Netrin 1 (Ntn1)

  Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)

  Instruction Instruction Instruction Instruction manual

  FOR IN VITRO USE AND RESEARCH USE ONLY

  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

  6th Edition (Revised in March, 2013)

  轴突生长诱向因子1(Ntn1)重组蛋白 PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]

  Residues: Residues: Residues: Residues: Pro313~Pro565 (Accession # O95631),

  with two N-terminal Tags, His-tag and GST-tags-Tag.

  Host: E. coli

  Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Secreted, extracellular

  space, extracellular matrix.

  Purity: Purity: Purity: Purity: >95%

  Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg

  (determined by the LAL method).

  Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,

  pH7.4, containing 5% sucrose, 0.01% sarcosyl.

  Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 9.0

  Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 55.5kDa

  Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

  (May be suitable for use in other assays to be determined by the end user.)

  轴突生长诱向因子1(Ntn1)重组蛋白[ USAGE ]

  Reconstitute in sterile PBS, pH7.2-pH7.4.

  [ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

  Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

  Store at 2-8oC for one month.

  Aliquot and store at -80oC for 12 months.

  Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target

  protein. The loss rate was determined by accelerated thermal degradation test,

  that is, incubate the protein at 37oC for 48h, and no obvious degradation and

  precipitation were observed. (Referring from China Biological Products Standard,

  which was calculated by the Arrhenius equation.) The loss of this protein is less

  than 5% within the expiration date under appropriate storage condition.

  [ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

  The target protein is fused with two N-terminal Tags, His-tag and GST-tags-Tag,

  its sequence is listed below.

  MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL

  DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD

  VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD

  GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQ

  GPLGSEF- PECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC RFNMELYKLS

  GRKSGGVC L N CR HNTAG RHC HYCKEGYYRD MGKPIT HRKA CKACDC HPVG

  AAGKTCNQTT GQCPCKDGVT GITCNRCAKG YQQSRSPIAP CIKIPVAPPT TAASSVEEPE

  DCDSYCKASK GKLKINMKKY CKKDYAVQIH ILKADKAGDW WKFTVNIISV YKQGTSRIRR

  GDQSLWIRSR DIACKCPKIK PLKKYLLLGN AEDSP