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STAT3 luciferase reporter plasmid （STAT3萤火虫荧光素酶报告基因质粒）
产品描述
STAT3-Luc荧光素酶报告质粒（报告基因质粒）（STAT3 luciferase reporter plasmid）是自主研发的用于检测STAT3转录活性水平为目的的报告基因。STAT3(signal transducers and activators of transduction-3)报告基因与多种恶性肿瘤的发生、发展和侵袭转移关系密切。
STAT3报告基因主要用于监控细胞内的STAT3转录活性、肿瘤****的研发以及基因过表达和RNAi的表型分析等。
pGMSTAT3-Lu是改造后的哺乳动物真核表达载体，在其多克隆位点插入了多个STAT3结合位点，可以高灵敏度地检测STAT3的激活水平。同时，对载体中预测出的其它转录因子以外的结合位点进行了适当的突变，增加了质粒的转录因子结合特异性。由于质粒体积减小，使得STAT3报告基因更易于转染。
STAT3 luciferase reporter plasmid （STAT3萤火虫荧光素酶报告基因质粒）质粒图谱
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STAT3 luciferase reporter plasmid （STAT3萤火虫荧光素酶报告基因质粒）运输与保存方法
冰袋（wet ice）运输。收到产品后放到-20 ºC保存。
STAT3 luciferase reporter plasmid （STAT3萤火虫荧光素酶报告基因质粒）注意事项
本质粒未经允许不得用于任何商业用途，也不得移交给订货人实验室以外的任何人或单位。
为了您的健康，实验操作时请穿实验服和带一次性手套。
使用说明
pGMSTAT3-Lu可以采用常规转染方法转染哺乳动物细胞。用荧光素酶检测试剂盒或双荧光素酶检测试剂盒进行检测。
STAT3 luciferase reporter plasmid （STAT3萤火虫荧光素酶报告基因质粒）参考文献
[1] Kang Y, et al. The radio-sensitizing effect of xanthohumol is mediated by STAT3 and EGFR suppression in doxorubicin-resistant MCF-7 human breast cancer cells. Biochim Biophys Acta. 1830(3):2638-48 (2013).
[2] Teng J, et al. HBx-dependent activation of Twist mediates STAT3 Control of epithelium-mesenchymal transition of liver cells. J Cell Biochem. 114(5):1097-104 (2013).
[3] Villa S, et al. The influence of the substitution pattern on the molecular conformation of ureido-1,2,5-oxadiazoles, related to STAT3 inhibitors: chemical behavior and structural investigation. ChemBiodivers. 9(7):1240-53 (2012).
[4] Han L, et al. MicroRNA-21 expression is regulated by β-catenin/STAT3 pathway and promotes glioma cell invasion by direct targeting RECK. CNS NeurosciTher. 18(7):573-83 (2012).
[5] Kwon MJ, et al. Protein kinase C-θ promotes Th17 differentiation via upregulation of Stat3. J Immunol. 188(12):5887-97 (2012).
[6] Seo HS, et al. Apigenin induces apoptosis via extrinsic pathway, inducing p53 and inhibiting STAT3 and NFκB signaling in HER2-overexpressing breast cancer cells. Mol Cell Biochem. 366(1-2):319-34 (2012).
[7] Lim EJ, et al. Methylsulfonylmethane suppresses breast cancer growth by down-regulating STAT3 and STAT5b pathways. PLoS One. 7(4):e33361 (2012).
[8] Hahm ER, et al. Sulforaphane inhibits constitutive and interleukin-6-induced activation of signal transducer and activator of transcription 3 in prostate cancer cells. Cancer Prev Res (Phila). 3(4):484-94 (2010).
[9] Lin L, et al. A novel small molecule, LLL12, inhibits STAT3 phosphorylation and activities and exhibits potent growth-suppressive activity in human cancer cells. Neoplasia. 12(1):39-50 (2010).
[10] Shin DS, et al. Cryptotanshinone inhibits constitutive signal transducer and activator of transcription 3 function through blocking the dimerization in DU145 prostate cancer cells. Cancer Res. 69(1):193-202 (2009).
[11] Duan Z, et al. Signal transducers and activators of transcription 3 pathway activation in drug-resistant ovarian cancer. Clin Cancer Res. 12(17):5055-63
