产品名称：Anti-His tag/FITC抗体

Anti-His tag/FITC抗体

产品编号YB-0287R-FITC

英文名称Anti-His tag/FITC

中文名称FITC标记的聚组氨酸抗体标签抗体

别    名HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.  

Anti-His tag/FITC抗体

说 明 书100ul  

产品类型标签抗体

研究领域肿瘤  细胞生物  **学  染色质和核信号  信号转导  合成与降解  结合蛋白  细胞类型标志物  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应 fusionprotein,HHHHHHtagged

产品应用IF=1:50-200  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

Anti-His tag/FITC抗体性    状Lyophilized or Liquid

浓    度1mg/ml

免 疫 原Synthetic peptide: HHHHHH conjugated to KLH

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-His tag/FITC抗体

产品介绍background:

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Important Note:

This product as supplied is intended for research use only,Anti-His tag/FITC抗体 not for use in human, therapeutic or diagnostic applications.

标签抗体

蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白

1） 6xHis与不同的Sepharose纯化填料结合（捆绑），可不受构象的影响，在天然或变形的条件下可进行进一步纯化。

2） 可在温和的洗脱液环境中，其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。

3） 6xHis标签比普通（Tag）要小得多，经纯化后的蛋白可直接进行下游操作。可用于任何表达系统（包括：

**，杆状病毒和哺乳动物）

4） 6xHis标签在生理溶液pH下不带电荷，其标签不会干涉重组蛋白的结构和功能， 6xHis不会影响蛋白分泌

5） 6xHis标签**原性差，重组蛋白可以不必除去标签而直接作为抗原进行**