产品名称：Anti-GFAP/FITC抗体

Anti-GFAP/FITC抗体

产品编号YB-0199R-FITC

英文名称Anti-GFAP/FITC

中文名称FITC标记的胶质纤维酸性蛋白抗体

别    名Astrocyte; FLJ45472; GFAP; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN.  

Anti-GFAP/FITC抗体

说 明 书100ul  

研究领域肿瘤  细胞生物  **学  神经生物学  信号转导  干细胞  细胞粘附分子  细胞类型标志物  细胞骨架  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,

产品应用Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:50-200  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量48kDa

Anti-GFAP/FITC抗体性    状Lyophilized or Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human GFAP

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-GFAP/FITC抗体

产品介绍background:

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Function:

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Subunit:

Interacts with SYNM. Isoform 3 interacts with PSEN1 (via N-terminus).

Subcellular Location:

Cytoplasm. Note=Associated with intermediate filaments.

Tissue Specificity:

Expressed in cells lacking fibronectin.

Post-translational modifications:

Phosphorylated by PKN1.

DISEASE:

Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Anti-GFAP/FITC抗体Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or ***** forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.

Similarity:

Belongs to the intermediate filament family.

Database links:

Entrez Gene: 281189 Cow

Entrez Gene: 2670 Human

Entrez Gene: 14580 Mouse

Entrez Gene: 24387 Rat

Omim: 137780 Human

SwissProt: Q28115 Cow

SwissProt: P14136 Human

SwissProt: P03995 Mouse

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

星形胶质细胞标志物 （Astrocyte Marker）

GFAP是一个56kDa的中间丝蛋白（intermediate filament，IF），在**神经系统发育期是一个特异性的标志物，以区别星形细胞和其它胶质细胞。GFAP表达在皮层和海马,急、慢性皮质酮**时表达减少。

GFAP可以和人、大鼠、小鼠的GFAP反应，在正常和肿瘤性的星形胶质细胞阳性表达，而神经节细胞、神经元、成纤维细胞、少突胶质细胞和这些细胞来源的肿瘤细胞阴性表达，主要用于星形胶质瘤等**神经系统肿瘤的诊断和鉴别诊断,GFAP的缺乏可导致AD病。