产品名称：Anti-GAPDH/FITC抗体

Anti-GAPDH/FITC抗体

产品编号YB-0459R-FITC

英文名称Anti-GAPDH/FITC

中文名称FITC标记的3-磷酸甘油醛脱氢酶（兔来源**组化用抗体）

别    名Glyceraldehyde-3-phosphate dehydrogenase; 38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein; BARS-38; cb609; EC 1.2.1.12; G3PD; G3PDH; GAPD; Glyceraldehyde 3 phosphate dehydrogenase; Glyceraldehyde 3 phosphate dehydrogenase liver; Glyceraldehyde 3 phosphate dehydrogenase muscle; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS, p38 component; OCT1 coactivator in S phase, 38-KD component; wu:fb33a10; G3P_HUMAN.  

Anti-GAPDH/FITC抗体

说 明 书100ul  

研究领域神经生物学  信号转导  新陈代谢  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应 Human, Mouse, Rat, Monkey,

产品应用IF=1:50-200  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量37kDa

细胞定位细胞膜

Anti-GAPDH/FITC抗体性    状Lyophilized or Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human GAPDH

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-GAPDH/FITC抗体

产品介绍background:

(GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, thereversible oxidative phosphorylation of glyceraldehyde-3-phosphatein the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Subunit:

Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation (By similarity). Interacts with EIF1AD, USP25, PRKCI and WARS.

Subcellular Location:

Cytoplasm, cytosol. Nucleus. Cytoplasm, Anti-GAPDH/FITC抗体perinuclear region. Membrane. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Postnuclear and Perinuclear regions.

Similarity:

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Database links:

Entrez Gene: 493876 Cat

Entrez Gene: 374193 Chicken

Entrez Gene: 403755 Dog

Entrez Gene: 2597 Human

Entrez Gene: 100042025 Mouse

Entrez Gene: 14433 Mouse

Entrez Gene: 396823 Pig

Entrez Gene: 100009074 Rabbit

Entrez Gene: 24383 Rat

Entrez Gene: 685186 Rat

Entrez Gene: 380259 Xenopus laevis

Entrez Gene: 448356 Xenopus tropicalis

Entrez Gene: 317743 Zebrafish

Omim: 138400 Human

SwissProt: Cat

SwissProt: P00356 Chicken

SwissProt: Q28259 Dog

SwissProt: P70685 Guinea pig

SwissProt: P04406 Human

SwissProt: P16858 Mouse

SwissProt: P00355 Pig

SwissProt: P46406 Rabbit

SwissProt: P04797 Rat

SwissProt: P51469 Xenopus laevis

SwissProt: Q5XJ10 Zebrafish

Unigene: 544577 Human

Unigene: 592355 Human

Unigene: 598320 Human

Unigene: 304088 Mouse

Unigene: 309092 Mouse

Unigene: 317779 Mouse

Unigene: 343110 Mouse

Unigene: 392463 Mouse

Unigene: 392480 Mouse

Unigene: 414470 Mouse

Unigene: 458138 Mouse

Unigene: 458416 Mouse

Unigene: 475698 Mouse

Unigene: 129558 Rat

Unigene: 91450 Rat

Unigene: 995 Xenopus laevis

Unigene: 35640 Zebrafish

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.