产品名称：Anti-ILK-1/FITC抗体

Anti-ILK-1/FITC抗体

产品编号YB-0317R-FITC

英文名称Anti-ILK-1/FITC

中文名称FITC标记的整合素连接激酶-1抗体

别    名59 kDa serine/threonine protein kinase; 59 kDa serine/threonine-protein kinase; ILK-2; ILK_HUMAN; Integrin linked Kinase; Integrin-linked protein kinase; DKFZp686F1765; EC 2.7.11.1; ILK 1; ILK 2; ILK; ILK1; ILK2; Integrin linked Kinase 2; Integrin linked protein kinase; p59; p59ILK.  

Anti-ILK-1/FITC抗体

说 明 书100ul  

研究领域肿瘤  神经生物学  信号转导  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow,

产品应用IF=1:50-200  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量50kDa

细胞定位细胞膜

Anti-ILK-1/FITC抗体性    状Lyophilized or Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human ILK-1 (351-400aa)

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-ILK-1/FITC抗体

产品介绍background:

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin linked kinase (ILK), interacts with the cytoplasmic domain of beta 1 Integrin. ILK encodes a predicted 451 amino acid protein, with an apparent molecular weight of 59 kDa. The ILK protein is a serine/threonine protein kinase with 4 ankyrin like repeats. ILK regulates integrin mediated signal transduction.

The ILK protein is important in different biological pathways such as cell adhesion, anchorage-dependent cell cycle progression, oncogenic transformation, and growth factor signaling. The kinase activity of ILK is low in non-activated cells; its activity is stimulated by cell-ECM interactions and by certain growth factors. 3 Negative regulation of ILK is mediated by two phosphatases: PTEN, a tumor suppressor lipid sphatase, and ILKAP, a PP2C protein phosphatase. In tumor cells that do not express PTEN protein, ILK is constitutively active.

Function:

Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.

Subunit:

Interacts with cytoplasmic domain of beta 1 subunit of integrin. Anti-ILK-1/FITC抗体Could also interacts with beta 2, beta 3 and/or beta 5 subunit of integrin. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with parvins and probably TGFB1I1. Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1.

Subcellular Location:

Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Tissue Specificity:

Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.

Post-translational modifications:

Autophosphorylated on serine residues.

Similarity:

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Contains 5 ANK repeats.

Contains 1 protein kinase domain.

Database links:

Entrez Gene: 3611 Human

Entrez Gene: 16202 Mouse

Entrez Gene: 170922 Rat

Omim: 602366 Human

SwissProt: Q13418 Human

SwissProt: O55222 Mouse

SwissProt: Q99J82 Rat

Unigene: 5158 Human

Unigene: 706355 Human

Unigene: 274846 Mouse

Unigene: 95042 Rat

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

ILK 是一种新发现的Ser/Thr蛋白激酶。ILK能够通过与整合素β1亚单位的结合介导细胞与胞外基质的连接，以依赖于PI3K的方式激活，并通过磷酸化下游底物PKB/AKT,GSK3等胞外信号的一项下游传递，对细胞的生长，分化，迁移等进行调控。由于ILK在胞内外信号传导中起着重要的作用。并且抑制ILK的活性能够导致细胞周期的停滞和细胞程序性死亡的启动，使其成为肿瘤**和肿瘤**的理想靶位点。

该蛋白也表达与肾小球系膜细胞，正常足细胞ILK的高水平表达对足细胞功能起着重要的生理作用。DN 患者ILK表达明显增加 ，暴露于高血糖的肾小球膜细胞的ILK 水平明显升高, ILK 与整合素和其他连接蛋白都受到高血糖的调节,并且促进DN 患者肾小球基质的沉积。