产品名称：Anti-GNB1/FITC抗体

Anti-GNB1/FITC抗体

产品编号YB-0348R-FITC

英文名称Anti-GNB1/FITC

中文名称FITC标记的G蛋白/鸟苷酸结合蛋白抗体

别    名G protein beta subunit GI/GS/GT; guanine nucleotide binding protein (G protein), beta polypeptide 1; guanine nucleotide-binding protein G; GBB1_HUMAN.  

Anti-GNB1/FITC抗体

说 明 书100ul  

研究领域细胞生物  **学  神经生物学  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,

产品应用IF=1:50-200  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量37kDa

Anti-GNB1/FITC抗体性    状Lyophilized or Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human G protein beta subunit GI

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-GNB1/FITC抗体

产品介绍background:

G protein beta subunit GI/GS/GT (guanine nucleotide-binding protein G). WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.

Function:

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Subunit:

G proteins are composed of 3 units, alpha, beta and gamma.Anti-GNB1/FITC抗体 Interacts with ARHGEF18 and RASD2. The heterodimer formed by GNB1 and GNG2 interacts with PTH1R (via C-terminus).

Post-translational modifications:

Phosphorylation at His-266 by NDKB contributes to G protein activation by increasing the high energetic phosphate transfer onto GDP.

Similarity:

Belongs to the WD repeat G protein beta family.

Contains 7 WD repeats.

Database links:

Entrez Gene: 2782 Human

Entrez Gene: 14688 Mouse

Entrez Gene: 24400 Rat

Omim: 139380 Human

SwissProt: P62873 Human

SwissProt: P62874 Mouse

SwissProt: P54311 Rat

Unigene: 430425 Human

Unigene: 2344 Mouse

Unigene: 126047 Rat

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

G蛋白又称：鸟苷酸结合蛋白，存在于全身各个组织和细胞中，G蛋白在信息传递中起到很重要的作用,参与细胞内外信息的相互传导。

**受体与腺苷酸环化酶是细胞膜上两类分开的蛋白质。**受体结合的部分在细胞膜的外表面，而腺苷环化酶在膜的胞浆面，在两者之间存在一种起耦联作用的调节蛋白——鸟苷酸结合蛋白（guanine nucleotide-binding regulatory protein）细胞受到各种刺激信息，包括化学性的**和神经递质及非化学性的声音、图像等。刺激信息通过G蛋白的激活作用，在胞浆中生成数目众多的**信使分子，产生十万级放大效应，引起细胞兴奋，让后促动器官完成各自功能。

1977年，美国科学家阿尔弗雷德-戈德曼-吉尔曼(Alfred Goodman Gilman)发现了G蛋白及其在细胞兴奋传导方面的重要作用。1994年，吉尔曼因“G蛋白”方面的研究成果而荣获诺贝尔生理及医学奖。