产品名称：Anti-E2F1/FITC抗体

Anti-E2F1/FITC抗体

产品编号YB0599R-FITC

英文名称Anti-E2F1/FITC

中文名称FITC标记的转录因子E2F-1抗体

别    名E2F 1; E2F transcription factor 1; E2F-1; E2f1 E2F transcription factor 1; KIAA4009; mKIAA4009; OTTHUMP00000030661; PBR 3; PBR3; PRB binding protein E2F 1; PRB-binding protein E2F-1; RBAP 1; RBAP-1; RBAP1; RBBP 3; RBBP-3; RBBP3; RBP 3; RBP3; Retinoblastoma associated protein 1; Retinoblastoma binding protein 3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1; E2F1_HUMAN.  

Anti-E2F1/FITC抗体

说 明 书100ul  

研究领域肿瘤  转录调节因子  表观遗传学  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应 Human, Mouse, Rat,

产品应用Flow-Cyt=1:50-200 ICC=1:50-200 IF=1:50-200  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量46kDa

Anti-E2F1/FITC抗体性    状Lyophilized or Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human E2F1

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-E2F1/FITC抗体

产品介绍background:

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]

Function:

Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.

Subunit:

Component of the DRTF1/E2F transcription factor complex. Anti-E2F1/FITC抗体Forms heterodimers with DP family members. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1. Interacts with ARID3A. Binds EAPP.

Subcellular Location:

Nucleus.

Post-translational modifications:

Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.

Similarity:

Belongs to the E2F/DP family.

Database links:

Entrez Gene: 1869 Human

Entrez Gene: 13555 Mouse

Entrez Gene: 399489 Rat

Omim: 189971 Human

SwissProt: Q01094 Human

SwissProt: Q61501 Mouse

SwissProt: O09139 Rat

Unigene: 654393 Human

Unigene: 18036 Mouse

Unigene: 72471 Rat

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

转录调节因子（Transcriptin Regulators）

E2F1—属于调节性转录因子E2F家族。有学者认为：E2F-1既可作为癌基因起作用，又可作为抑癌基因起作用。其不同可能由细胞中其他生长促进或抑制性蛋白质水平和（或）活性决定，同时与细胞所处环境及器官特异性有关。在控制细胞周期和肿瘤抑制基因蛋白的活性方面起关键作用。