产品名称：Anti-RUNX2/FITC抗体

Anti-RUNX2/FITC抗体

产品编号YB-1134R-FITC

英文名称Anti-RUNX2/FITC

中文名称FITC标记的核心结合因子α1/成骨特异性转录因子/Cbfα1抗体

别    名RUNX2_HUMAN; Runt-related Transcription Factor 2; CBF alpha 1; CBF-alpha-1; PEBP2-alpha A; CBFA1; CCD; CCD1; Cleidocranial dysplasia 1; Core binding factor; Core binding factor runt domain alpha subunit 1; Core binding factor subunit alpha 1; MGC120023; Oncogene AML 3; OSF 2; OSF2; OSF-2; Osteoblast specific transcription factor 2; OTTHUMP00000016533; PEA2 alpha A; PEA2aA; PEBP2 alpha A; PEBP2A1; PEBP2A2; PEBP2aA1; Polyomavirus enhancer binding protein 2 alpha A subunit; Runt domain; Runt related transcription factor 2; SL3 3 enhancer factor 1 alpha A subunit; SL3/AKV core binding factor alpha A subunit; AML3; CLCD.  

Anti-RUNX2/FITC抗体

研究领域干细胞  转录调节因子  表观遗传学  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,

产品应用IF=1:50-200  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量57(hu)/67(mo,rat)kDa

Anti-RUNX2/FITC抗体性    状Lyophilized or Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human RUNX2

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-RUNX2/FITC抗体

产品介绍background:

This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2008].

Function:

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation. [SUBUNIT] Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors (By similarity). Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 (By similarity). The alpha subunit binds DNA as Anti-RUNX2/FITC抗体a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with MYST3 and MYST4.

Subunit:

Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with G22P1 (Ku70) and XRCC5 (Ku80). Interacts with MYST3 and MYST4.

Subcellular Location:

Nucleus.

Tissue Specificity:

Specifically expressed in osteoblasts.

Post-translational modifications:

Phosphorylated; probably by MAP kinases (MAPK). Isoform 3 is phosphorylated on Ser340.

DISEASE:

Defects in RUNX2 are the cause of cleidocranial dysplasia (CLCD) [MIM:119600]; also known as cleidocranial dysostosis (CCD). CLCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies.

Similarity:

Contains 1 Runt domain.

Database links:

Entrez Gene: 860 Human

Entrez Gene: 12393 Mouse

Entrez Gene: 100155806 Pig

Entrez Gene: 367218 Rat

Omim: 600211 Human

SwissProt: Q13950 Human

SwissProt: Q9XSB7 Horse

SwissProt: Q08775 Mouse

SwissProt: Q9Z2J9 Rat

Unigene: 535845 Human

Unigene: 391013 Mouse

Unigene: 391017 Mouse

Unigene: 214214 Rat

Unigene: 83672 Rat

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

RUNX2又称：Cbfα1(Core-binding factor, alpha 3 subunit) 是新发现的一类调控间充质干细胞向成骨方向分化的特异性转录因子,参与骨形成,骨骼生长和发育的一类重要细胞,它起源于多能间充质干细胞,是间充质干细胞在体内的各种调控因素的调节下发育而成的。