产品名称：Anti-E2F2/FITC抗体

Anti-E2F2/FITC抗体

产品编号YB1001R-FITC

英文名称Anti-E2F2/FITC

中文名称FITC标记的转录因子E2F-2抗体

别    名dE2F2; E2F-2; E2F2; E2F2_HUMAN; Transcription factor E2F2; E2F transcription factor 2.  

Anti-E2F2/FITC抗体

说 明 书100ul  

研究领域**学  染色质和核信号  表观遗传学  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Horse,

产品应用IF=1:50-200  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量48kDa

Anti-E2F2/FITC抗体性    状Lyophilized or Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human E2F2 N-terminus

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-E2F2/FITC抗体

产品介绍background:

E2F/DP family winged-helix DNA-binding domain. This family contains the transcription factor E2F and its dimerisation partners TDP1 and TDP2, which stimulate E2F-dependent transcription. E2F binds to DNA as a homodimer or as a heterodimer in association with TDP1/2, the heterodimer having increased binding efficiency. The crystal structure of an E2F4-DP2-DNA complex shows that the DNA-binding domains of the E2F and DP proteins both have a fold related to the winged-helix DNA-binding motif. Recognition of the central c/gGCGCg/c sequence of the consensus DNA-binding site is symmetric, and amino acids that contact these bases are conserved among all known E2F and DP proteins.

Function:

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.

Subunit:

Component of the DRTF1/E2F transcription factor complex.Anti-E2F2/FITC抗体 Forms heterodimers with DP family members. The E2F2 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB1, thus releasing the active complex. Binds EAPP.

Subcellular Location:

Nucleus.

Tissue Specificity:

Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.

Post-translational modifications:

Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase (By similarity).

Similarity:

Belongs to the E2F/DP family.

Database links:

Entrez Gene: 1870 Human

Entrez Gene: 242705 Mouse

Omim: 600426 Human

SwissProt: Q14209 Human

SwissProt: P56931 Mouse

Unigene: 194333 Human

Unigene: 307932 Mouse

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

转录调节因子（Transcriptin Regulators）

E2F2(又称：TFE-25)—属于调节性转录因子E2F家族。