产品名称：Anti-eIF4A1/FITC抗体

Anti-eIF4A1/FITC抗体

产品编号YB-33142M-FITC

英文名称Anti-eIF4A1/FITC

中文名称FITC标记的eIF4A1蛋白单克隆抗体

别    名ATP-dependent RNA helicase eIF4A-1; DDX2; DDX2A; eIF 4A I; eIF-4A-I; EIF4A; eIF4A I; eIF4A-I; EIF4A1; Eukaryotic initiation factor 4A-I; Eukaryotic initiation factor 4AI; eukaryotic translation initiation factor 4A, isoform 1; Eukaryotic translation initiation factor 4A1; IF4A1_HUMAN.  

Anti-eIF4A1/FITC抗体

说 明 书100ug  

研究领域细胞生物  表观遗传学  

抗体来源Mouse

克隆类型Monoclonal

克 隆 号1B8

交叉反应

产品应用ICC=1:50-200 IF=1:50-200  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量46kDa

Anti-eIF4A1/FITC抗体性    状Lyophilized or Liquid

浓    度2mg/1ml

免 疫 原KLH conjugated synthetic peptide derived from human eIF4A1

亚    型IgG

纯化方法affinity purified by Protein G

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-eIF4A1/FITC抗体

产品介绍background:

Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a 5’-cap motif and recruits the 43S ribosomal preinitiation complex to the eligible transcript. Along with eIF4B, the eIF4F complex mediates the unwinding of mRNA secondary structure to facilitate ribosome association. eIF4E specifically interacts with the 5’ cap, eIF4A(I,II) are bidirectional RNA helicases, and eIF4G(I,II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3 and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92.7% homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2.

Function:

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation,Anti-eIF4A1/FITC抗体 eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Subunit:

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with PAIP1, EIF4E and UPF2. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact with NOM1. Interacts with PDCD4; this interferes with the interaction between EIF4A and EIF4G. Interacts with RBM4. Interacts with human cytomegalovirus/HHV-5 protein UL69.

Similarity:

Belongs to the DEAD box helicase family. eIF4A subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Database links:

Entrez Gene: 1973 Human

Entrez Gene: 504958 Cow

Entrez Gene: 13681 Mouse

Entrez Gene: 287436 Rat

Omim: 602641 Human

SwissProt: Q3SZ54 Cow

SwissProt: P60842 Human

SwissProt: P60843 Mouse

Unigene: 129673 Human

Unigene: 279821 Mouse

Unigene: 371557 Mouse

Unigene: 91523 Rat

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.