Repligen Corporation 特约代理 品牌logo 公司网址：
http://www.repligen.com/ Repligen is a bioprocessing-focused life sciences company bringing over 30 years of expertise and innovation to our customers. We are inspiring advances in bioprocessing through the development and commercialization of high-value products and flexible solutions that address critical steps in the production of biologic drugs, principally monoclonal antibodies. Our core Protein A affinity ligands business is complemented by an expanding suite of technology-leading bioprocessing products that we sell direct to biopharmaceutical companies and contract manufacturers worldwide. All of our bioprocessing products are meaningfully differentiated from alternatives to the benefit of our customers. Our Protein A ligands are used to purify over 50 monoclonal antibody-based drugs on the market and more than 300 in clinical development. Our XCell™ ATF cell concentration device, and cell culture supplements are at the forefront of innovation in bioprocessing. To provide customers with the broadest selection of pre-packed chromatography columns from bench-scale to production-scale, Repligen acquired Atoll GmBH of Weingarten, Germany, solidifying Repligen as a leading supplier in this fast-growing segment. 产品列表： XCell™ ATF System - Stainless Steel XCell™ ATF Cell Retention System The XCell™ ATF System in stainless steel comes in five different sizes for operation from lab-scale to production-scale. Each system consists of a diaphragm pump in a stainless steel housing, a single-use hollow fiber filter cartridge, and a control system. •XCell™ ATF 2 System •XCell™ ATF 4 System •XCell™ ATF 6 System •XCell™ ATF 8 System •XCell™ ATF 10 System XCell™ ATF System - Stainless Steel Technology The XCell™ ATF System is based on award-winning Alternating Tangential Flow (XCell™ ATF) technology, created by the action of a diaphragm moving upward and downward within a pump head, connected to a filter housing and attached to a bioreactor. Alternating tangential flow is attained by the action of a diaphragm pump. XCell™ atf technology Benefits ATF Cell Retention System •Continuous, provides clarified harvest ready for capture chromatography •Efficient, delivers 100% cell retention, increases productivity •Intensified, generates high cell concentration and viability •Flexible, connects to any bioreactor and can be used in a variety of applications •Gentle, reduces cell stress, increases cell viability and productivity •Easy to use, reduces setup time and user training •Scalable, adapts from development-scale to production-scale •Self-cleaning, reduces biofilm build up on the hollow fiber filter Applications ATF Cell Retention System Perfusion Perfusion, or upstream continuous processing, has been used since the 1980s. High cell density perfusion, or ATF perfusion, delivers higher cell concentration and cell viability. N-1 Perfusion N-1 perfusion refers to the intensification of cell growth in the step prior to the production bioreactor (N). This process intensification is done using perfusion in the N-1 bioreactor for high cell density seeding of the production bioreactor (N), or removal of the N-1 bioreactor if the N-2 bioreactor can provide enough cells for the production bioreactor (N). High Density (HD) Cell Banking High density (HD) cell banking is a novel method for seed train optimization using perfusion to intensify cell density and reduce the number of early-stage expansion steps. Learn more Connecting to a bioreactor The XCell™ ATF System is easy to connect to standard bioreactors, including •Glass bioreactors (1L - 20L) •Wave type single-use bioreactor (1L - 1000L) •Stirred tank single-use bioreactor (2L - 2000L) •Stainless steel bioreactors (20L - 2000L) A complete guide includes the range of standard adaptors and ports that fit all bioreactor equipment. SEE GUIDE Resources LONG®R3 IGF-I Cell Culture Supplement LONG® EGF Cell Culture Supplement Alternating Tangential Flow (XCell™ ATF) technology XCell™ ATF Cell Retention System The XCell™ ATF System integrates a diaphragm pump, a stainless steel housing, and a single-use hollow fiber filter cartridge. •Connect the XCell™ ATF System to a bioreactor. •Pressurized air flows into the bottom of the ATF pump. •The air pressure forces the diaphragm gently up, displacing liquid and creating a fast flow of cell culture media across the surface of the hollow fiber filter. •There is rapid flow of media through the filter fibers, with a cell-free filtrate being generated. •The cell culture fluid remaining on the upstream side of the hollow fiber filter is returned back into the bioreactor.
