产品名称：人软脑膜周细胞

收到人软脑膜周细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

人软脑膜周细胞

Central nervous system pericytes are perivascular cells that are closely associated with the endothelium of capillaries and other small vessels [1].  Pericytes, located near endothelial cells, communicate with other cells by extending long cytoplasmic processes which wrap around the capillaries [1, 2]. Pericytes participate in a variety of processes including angiogenesis, endothelial cell survival, regulation of capillary blood flow, and establishment and maintenance of the blood-brain barrier [3, 4]. Pericyte dysregulation has been linked to several pathological conditions such as hypertension, diabetic retinopathy, atherosclerosis, multiple sclerosis, Alzheimer’s disease, and tumor angiogenesis [2, 4].  The unique and diverse functions of pericytes make them novel candidates for cell therapy in regenerative medicine. Leptomeningeal pericytes (LP) are derived from the leptomeningeal layer surrounding the brain. Cultured human leptomeningeal pericytes (HLP) are a useful in vitro model for studying a wide variety of central nervous system diseases.

HLP from ScienCell Research Laboratories are isolated from human brain. HLP are cryopreserved at passage one after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HLP are characterized by immunofluorescence with antibody specific to a-smooth muscle actin and platelet derived growth factor-b (PDGF-b). HLP are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HLP are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium:

It is recommended to use Pericyte Medium (PM, Cat. #1201) for culturing HLP in vitro.

Catalog No. 1410

Country of Manufacture United States

Product Code HLP

Size/Quantity 5 x 10^5 cells/vial

Product Use HLP are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.

Shipping Info Dry ice.

References [1] Dore-Duffy P, Cleary K. (2011) "Morphology and properties of pericytes." Methods Mol Biol. 686:49-68.

[2] Allt G, Lawrenson JG. (2001) "Pericytes: cell biology and pathology." Cells Tissues Organs. 169: 1-11.

[3] Daneman R, Zhou L, Kebede A, Barres B. (2010) "Pericytes are required for blood-brain barrier integrity during embryogenesis" Nature. 468:562-566.

[4] Kutcher M, Herman I. (2009) "The pericyte: cellular regulator of microvascular blood flow." Microvasc Res.

人软脑膜周细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，人软脑膜周细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
人软脑膜周细胞其它细胞系展示表：yb1260 MN-h 小鼠海马趾神经元 Mouse Neurons-hippocampal 1 x 10^6 cells/vial ATCC
yb1261 MN-vsc 小鼠腹脊髓神经元 Mouse Neurons-ventral spinal cord 1 x 10^6 cells/vial ATCC
yb1262 MN-dsc] 小鼠背脊髓神经元 Mouse Neurons-dorsal spinal cord 1 x 10^6 cells/vial ATCC
yb1263 MN-vsc 小鼠腹脊髓神经元 Mouse Neurons-ventral spinal cord 1 x 10^6 cells/vial ATCC
yb1264 MSC 小鼠雪旺细胞 Mouse Schwann Cells 5 x 10^5 cells/vial ATCC
yb1265 MA 小鼠星形胶质细胞 Mouse Astrocytes 5 x 10^5 cells/vial ATCC
yb1266 MA-c 小鼠小脑星形胶质细胞 Mouse Astrocytes-cerebellar 5 x 10^5 cells/vial ATCC
yb1267 MA-h 小鼠海马趾星形胶质细胞 Mouse Astrocytes-hippocampal 5 x 10^5 cells/vial ATCC
yb1268 MA-sc 小鼠脊髓星形胶质细胞 Mouse Astrocytes-spinal cord 5 x 10^5 cells/vial ATCC
yb1269 MM 小鼠小胶质细胞 Mouse Microglia 5 x 10^5 cells/vial ATCC
yb1270 MMa-bm 小鼠巨噬细胞 Mouse Macrophage 1 x 10^6 cells/vial ATCC
yb1271 MLF 小鼠**成纤维细胞 Mouse Lymphatic Fibroblasts 5 x 10^5 cells/vial ATCC
yb1272 MSGF 小鼠唾腺成纤维细胞 Mouse Salivary Gland Fibroblasts 5 x 10^5 cells/vial ATCC
yb1273 MRTEpiC 小鼠肾小管上皮细胞 Mouse Renal Tubular Epithelial Cells 5 x 10^5 cells/vial ATCC
yb1274 MCM 小鼠心肌细胞 Mouse Cardiac Myocytes 1 x 10^6 cells/vial ATCC
yb1275 MCF 小鼠心脏成纤维细胞 Mouse Cardiac Fibroblasts 5 x 10^5 cells/vial ATCC
yb1276 MMSC-bm 小鼠骨髓间充质干细胞 Mouse Mesenchymal Stem Cells-bone marrow 5 x 10^5 cells/vial ATCC
yb1277 MEF-mt 小鼠胚胎成纤维细胞 Mouse Embryonic Fibroblasts 5 x 10^5 cells/vial ATCC
yb1278 ATCC
yb1279 大鼠细胞系统 ATCC
yb1280 RPC 大鼠垂体细胞 Rat Pituitary Cells 5 x 10^5 cells/vial ATCC
yb1281 RMC 大鼠脑膜细胞 Rat Meningeal Cells 5 x 10^5 cells/vial ATCC
yb1282 RN-r 大鼠脑脊神经元 Rat Neurons-raphe 1 x 10^6 cells/vial ATCC
yb1283 RN-c 大鼠皮质神经元 Rat Neurons-cortical 1 x 10^6 cells/vial ATCC
yb1284 RGC 大鼠颗粒细胞 Rat Granule Cells 1 x 10^6 cells/vial ATCC
yb1285 RN-h 大鼠海马趾神经元 Rat Neurons-hippocampal 1 x 10^6 cells/vial ATCC
yb1286 RN-sn 大鼠黑质神经元 Rat Neurons-substantia nigra 1 x 10^6 cells/vial ATCC