产品名称：小鼠巨噬细胞

收到小鼠巨噬细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠巨噬细胞Description

Macrophages are cells produced by the differentiation of monocytes in tissues. Macrophages are large cells found within many types of tissue. They derive from monocytes that have entered tissues. Macrophages phagocytise invading microorganisms and also scavenge dead and damaged cells and cellular debris. In the bone marrow and subsequestly in the blood and tissues, cells of this series undergo a series of functional and morphologic maturation steps that culminate in the mature tissue macrophage [1]. Macrogphage can be identified by specific expression of a number of proteins including CD14, CD11b, F4/80 (mice)/EMR1 (human), MAC-1/MAC-3 and CD68 by flow cytometory or immunohistochemical staining [2].

MMa-bm from ScienCell Research Laboratories are isolated from ***** mouse bone marrow. Cells are harvested after purification and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. MMa-bm is characterized by immunofluorescent method with antibody to F4/80. MMa-bm is negative for mycoplasma, bacteria, yeast and fungi. MMa-bm is not recommended for expanding or long term cultures since the cells do not proliferate in culture.

Recommended Medium

It is recommended to use Macrophage Medium (MaM, Cat. No. 1921) for the culturing of MMa-bm in vitro.

Product Use

MMa-bm are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

Reference

[1]. Martin Cline and Margaret Sumner (1972) Bone Marrow Macrophage Precursors. I. Some Functional Characteristics of the Early Cells of the Mouse Macrophage Series Blood 40(1) 62-69.

[2]. Siamon Gordon and Philip Taylor (2005) Monocyte and macrophage heterogeneity. Nature Reviews Immunology 5(12) 953-964.

小鼠巨噬细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，小鼠巨噬细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
小鼠巨噬细胞其它细胞系展示表：CNE 鼻咽癌 品牌ATCC 规格25毫升/株. 货期1-2周
Ho-8910 卵巢癌 品牌ATCC 规格25毫升/株. 货期1-2周
TT 髓性甲状腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
L1 卵巢癌 品牌ATCC 规格25毫升/株. 货期1-2周
Bcap-37 乳腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
HeLa 宫颈癌 品牌ATCC 规格25毫升/株. 货期1-2周
ZR-75-30 乳腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
HeLa229 宫颈癌 品牌ATCC 规格25毫升/株. 货期1-2周
MCF-7 乳腺腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
KB-C1.5 宫颈癌 品牌ATCC 规格25毫升/株. 货期1-2周
SK-BR-3 乳腺腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
HCC **高分化鳞癌 品牌ATCC 规格25毫升/株. 货期1-2周
MDA-MB-435S 乳腺管癌 品牌ATCC 规格25毫升/株. 货期1-2周
JAR 胎盘绒毛癌 品牌ATCC 规格25毫升/株. 货期1-2周
T47D 乳腺管癌 品牌ATCC 规格25毫升/株. 货期1-2周
RD 恶性胚胎横纹肌瘤 品牌ATCC 规格25毫升/株. 货期1-2周
ybL-100 乳腺细胞 品牌ATCC 规格25毫升/株. 货期1-2周
BT-325 神经胶质瘤 品牌ATCC 规格25毫升/株. 货期1-2周
SMC-1 恶性胸膜间皮瘤 品牌ATCC 规格25毫升/株. 货期1-2周
SK-N-SH 神经母细胞瘤 品牌ATCC 规格25毫升/株. 货期1-2周
A2 肺癌 品牌ATCC 规格25毫升/株. 货期1-2周
SHG-44 胶质瘤 品牌ATCC 规格25毫升/株. 货期1-2周
95-D 高转移肺癌 品牌ATCC 规格25毫升/株. 货期1-2周
A375 恶性黑色素瘤 品牌ATCC 规格25毫升/株. 货期1-2周
Calu-3 肺腺癌 （胸膜渗出液） 品牌ATCC 规格25毫升/株. 货期1-2周
Bowes Melanoma 黑色素瘤 品牌ATCC 规格25毫升/株. 货期1-2周
LTEP-a-2 肺腺癌 品牌ATCC 规格25毫升/株. 货期1-2周
MM96L 黑色素瘤 品牌ATCC 规格25毫升/株. 货期1-2周
SH-77 小细胞肺癌 品牌ATCC 规格25毫升/株. 货期1-2周
J-111 单核细胞白血病 品牌ATCC 规格25毫升/株. 货期1-2周