产品名称：小鼠脊髓星形胶质细胞

收到小鼠脊髓星形胶质细胞时如无异常情况，请在显微镜下观察细胞密度，如为贴壁细胞，未超过80%汇合度时，将培养瓶中培养液吸出，留下10ml培养液继续培养；超过80%汇合度时，请按细胞培养条件传代培养。如为悬浮细胞，吸出培养液、1000转/分钟离心2分钟，吸出上清，管底细胞用新鲜培养基悬浮细胞后移回培养瓶。

小鼠脊髓星形胶质细胞Description

Astrocytes are the major cell type in the mammalian brain. They have been implicated in a variety of supportive functions for their partner neurons in the CNS, such as neuronal guidance during development, ion and water homeostasis, blood flow regulation, neurotransmission, energy metabolism, and immune defense [1]. Recent studies have shown that spinal cord astrocytes contribute to neuroinflammation by chemokine expression which leads to the recruitment of “inflammatory” monocytes and neutrophils to the lesion site [2]. Experimentally, spinal cord astrocytes have also been used to study the wobbler mutation and muscular dystrophy in mice [3] and astrogliosis [4]. As the recognition of the importance of astrocytes in nervous system functioning is increasing, specifically regarding the modulation of neural activity, astrocyte cultures are continuing to provide a useful tool in exploring the diverse properties and functions of these cells.

MA-sc from ScienCell Research Laboratories are isolated from neonate day two mouse spinal cord. MA-sc are cryopreserved at primary culture and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. MA-sc are characterized by immunofluorescence with antibody specific to GFAP. MA-sc are negative for mycoplasma, bacteria, yeast and fungi. MA-sc are guaranteed to further expand for 5 population doublings in the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Astrocyte Medium-animal (AM-a, Cat. No. 1831) for the culturing of MA-sc in vitro.

Product Use

MA-sc are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

Reference

[1] Oberheim N, Goldman S, Nedergaard M. (2012) Heterogeneity of astrocytic form and function. Methods in Mol Biol. 814: 23-45.

[2] Pineau I, Sun L, Bastien D, Lacroix S. (2010) Astrocytes initiate inflammation in the injured mouse spinal cord by promoting the entry of neutrophils and inflammatory monocytes in an IL-1 receptor/MyD88-depdent fashion. Brain Behav Immun. 24: 540-53.

[3] Hantaz-Ambroise D, Blondet B, Murawsky M, Rieger F. (1994) Abnormal astrocyte differentiation and defective cellular interactions in wobbler mouse spinal cord. J Neurocytol. 23: 179-92.

[4] Bhalala O, Pan L, Sahni V, McGuire T, Gruner K, Tourtellotte W, Kessler J. (2012) microRNA-21 regulates astrocytic response following spinal cord injury. J Neurosci. 32: 17935-47.

小鼠脊髓星形胶质细胞传代方法：
 收到细胞后，取出培养瓶在倒置显微镜下观察细胞生长情况。
（一）如果细胞未长满，用75%酒精喷洒整个瓶**后放到超菌台内，严格无菌操作，打开细胞培养瓶，吸出培养液，仅留下10ml培养液在瓶内继续培养。
(二)如果细胞已长满，即可进行传代培养。具体步骤如下：
1. 弃去培养液，用PBS（不含钙，镁离子）洗1-2次。
2. 加1ml消化液（0.25%Trypsin-0.53mM EDTA）于培养瓶中，小鼠脊髓星形胶质细胞倒转放于37度培养箱1-3分钟预热，然后又将培养瓶倒转大约30秒后，在倒置显微镜下观察细胞消化情况，若细胞大部分变圆分散，轻敲几下培养培养瓶，细胞随即脱落下来。
3. 加入6-8ml完全培养基，吸出，分到新的培养瓶中。1:3~1:6传代；2~3天1次。 
。
注意：传代后一半用我们的培养基，一半用你们的，以免细胞不适应而造
成生长不好。
冻存方法：   冻存液：基础培养基+5%DMSO+20%FBS 
储存：液氮储存
小鼠脊髓星形胶质细胞其它细胞系展示表：yb-hxbz-015 人食管癌细胞 TE-11 规格25毫升/株.
yb-hxbz-016 人食管癌细胞 EC97076 规格25毫升/株.
yb-hxbz-017 人食管鳞癌 KYSE 150 规格25毫升/株.
yb-hxbz-018 人食管鳞癌 KYSE 450 规格25毫升/株.
yb-hxbz-019 人食管鳞癌 KYSE30 规格25毫升/株.
yb-hxbz-020 人食管鳞癌 KYSE70 规格25毫升/株.
yb-hxbz-021 人食管癌细胞 KYSR450 规格25毫升/株.
yb-hxbz-022 人食管癌细胞 KYSE510 规格25毫升/株.
yb-hxbz-023 人胃癌细胞系 HS-746T 规格25毫升/株.
yb-hxbz-024 人胃癌细胞 BSG823 规格25毫升/株.
yb-hxbz-025 人胃粘膜细胞 GES-1 规格25毫升/株.
yb-hxbz-026 人胃腺癌细胞 MGC-803 规格25毫升/株.
yb-hxbz-027 人胃腺癌细胞 MCG803 规格25毫升/株.
yb-hxbz-028 人低分化胃癌细胞 BGC-823 规格25毫升/株.
yb-hxbz-029 人低分化胃癌细胞 SGC7901 规格25毫升/株.
yb-hxbz-030 人耐VCR胃腺癌细胞 SGC-7901/VCR 规格25毫升/株.
yb-hxbz-031 人低分化胃癌细胞 MKN-45 规格25毫升/株.
yb-hxbz-032 人未分化胃癌细胞 HGC-27 规格25毫升/株.
yb-hxbz-033 人胃腺癌细胞 AGS 规格25毫升/株.
yb-hxbz-034 人胃腺癌细胞 NCI-N87 规格25毫升/株.
yb-hxbz-035 人胃癌细胞 MKN-28 规格25毫升/株.
yb-hxbz-036 人胃癌细胞 (+)9811 规格25毫升/株.
yb-hxbz-038 人胃癌细胞 9811C11 规格25毫升/株.
yb-hxbz-039 绿色荧光蛋白标记人胃癌细胞 MGC803-GFP 规格25毫升/株.
编号 中文 英文名称 规格25毫升/株.
yb-hxbz-040 人胃癌细胞 BGC-803 规格25毫升/株.
yb-hxbz-041 人胃癌顺铂耐药株 SGC7901/DDP 规格25毫升/株.