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着丝粒蛋白E(CENPE)重组蛋白
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 性状冻干粉内**水平<1.0EU/µg(LAL法测定)
- 亚细胞定位n/a
- 预测分子量20.2kDa
- 实际分子量-(差异分析请参阅说明书)
- 片段与标签Ala2450~Arg2590 (Accession # Q02224) with N-terminal His Tag
- 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 纯度> 95%
- 等电点-
-
应用SDS-PAGE; WB; ELISA; IP
着丝粒蛋白E(CENPE)重组蛋白用法
PBS或其它。
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
着丝粒蛋白E(CENPE)重组蛋白
Centromere Protein E (CENPE)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
着丝粒蛋白E(CENPE)重组蛋白[ PROPERTIES ]
Residues: Ala2450~Arg2590 (Accession # Q02224),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Chromosome, centromere,
kinetochore. Cytoplasm, cytoskeleton, spindle.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 9.4
Predicted Molecular Mass: 20.2kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
着丝粒蛋白E(CENPE)重组蛋白[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- A KPYKEEIEDL KMKLVKIDLE
KMKNAKEFEK EISATKATVE YQKEVIRLLR ENLRRSQQAQ DTSVISEHTD PQPSNKPLTC
GGGSGIVQNT KALILKSEHI RLEKEISKLK QQNEQLIKQK NELLSNNQHL SNEVKTWKER
