产品名称：组织转谷氨酰胺酶(TGM2)重组蛋白

组织转谷氨酰胺酶(TGM2)重组蛋白

• 物种Homo sapiens (Human，人)相同的名称，不同的物种。
• 性状冻干粉内**水平<1.0EU/µg(LAL法测定)
• 亚细胞定位n/a
• 预测分子量78.8kDa
• 实际分子量-(差异分析请参阅说明书)
• 片段与标签Met1~Ala687 (Accession # P21980) with N-terminal His Tag
• 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
• 纯度> 95%
• 等电点5.3
• 应用SDS-PAGE; WB; ELISA; IP
  组织转谷氨酰胺酶(TGM2)重组蛋白用法
  PBS或其它。
  储存
  避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
  稳定性
  热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
  
  组织转谷氨酰胺酶(TGM2)重组蛋白

  
  

  Recombinant Transglutaminase 2, Tissue (TGM2)

  Organism Species: Homo sapiens (Human)

  Instruction manual

  FOR IN VITRO USE AND RESEARCH USE ONLY

  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

  9th Edition (Revised in Jul, 2013)

  组织转谷氨酰胺酶(TGM2)重组蛋白[ PROPERTIES ]

  Residues: Met1~Ala687 (Accession # P21980),

  with N-terminal His-Tag.

  Host: E. coli

  Purity: >95%

  Endotoxin Level: <1.0EU per 1μg

  (determined by the LAL method).

  Formulation: Supplied as lyophilized form in PBS,

  pH7.4, containing 1mM DTT, 5% trehalose, 0.1%

  sarcosyl and preservative.

  Predicted isoelectric point: 5.3

  Predicted Molecular Mass: 78.8kDa

  Accurate Molecular Mass: 100kDa as determined by SDS-PAGE reducing conditions.

  Applications: SDS-PAGE; WB; ELISA; IP.

  (May be suitable for use in other assays to be determined by the end user.)

  Note: The possible reasons that the actual band size differs from the predicted are as follows:

  1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

  2. Relative charge: The composition of amino acids may affects the charge of the protein.

  3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

  4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

  give the active form.

  5. Polymerization of the target protein: Dimerization, multimerization etc.

  组织转谷氨酰胺酶(TGM2)重组蛋白[ USAGE ]

  Reconstitute in sterile PBS, pH7.2-pH7.4.

  [ STORAGE AND STABILITY ]

  Storage: Avoid repeated freeze/thaw cycles.

  Store at 2-8oC for one month.

  Aliquot and store at -80oC for 12 months.

  Stability Test: The thermal stability is described by the loss rate of the target

  protein. The loss rate was determined by accelerated thermal degradation test,

  that is, incubate the protein at 37oC for 48h, and no obvious degradation and

  precipitation were observed. (Referring from China Biological Products Standard,

  which was calculated by the Arrhenius equation.) The loss of this protein is less

  than 5% within the expiration date under appropriate storage condition.

  [ SEQUENCES ]

  The target protein is fused with N-terminal His-Tag, its sequence is listed below.

  MGHHHHHHSGSEF -MAEELVLERC DLELETNGRD HHTADLCREK LVVRRGQPFW

  LTLHFEGRNY EASVDSLTFS VVTGPAPSQE AGTKARFPLR DAVEEGDWTA TVVDQQDCTL

  SLQLTTPANA PIGLYRLSLE ASTGYQGSSF VLGHFILLFN AWCPADAVYL DSEEERQEYV

  LTQQGFIYQG SAKFIKNIPWNFGQFEDGIL DICLILLDVN PKFLKNAGRD CSRRSSPVYV

  GRVVSGMVNC NDDQGVLLGR WDNNYGDGVS PMSWIGSVDI LRRWKNHGCQ

  RVKYGQCWVF AAVACTVLRC LGIPTRVVTN YNSAHDQNSN LLIEYFRNEF GEIQGDKSEM

  IW N F HCWVES WM T RP D LQPG YEGWQA L DPT PQEKS EGTY C CGPV PV RAIK

  EGDLSTKYDA PFVFAEVNAD VVDWIQQDDG SVHKSINRSL IVGLKISTKS VGRDEREDIT

  HTYKYPEGSS EEREAFTRAN HLNKLAEKEE TGMAMRIRVG QSMNMGSDFD VFAHITNNTA

  EEYVCRLLLC ARTVSYNGIL GPECGTKYLL NLNLEPFSEK SVPLCILYEK YRDCLTESNL

  IKVRALLVEP VINSYLLAER DLYLENPEIK IRILGEPKQK RKLVAEVSLQ NPLPVALEGC

  TFTVEGAGLT EEQKTVEIPD PVEAGEEVKV RMDLLPLHMG LHKLVVNFES DKLKAVKGFR

  NVIIGPA

  [ REFERENCES ]

  1. Gentile V., et al. (1991) J. Biol. Chem. 266:478-483. 2. Fraij B.M., et al. (1992) J. Biol. Chem. 267:22616-22623. 3. Van Damme P., et al. (2012) Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454. 4. Pinkas D.M., et al. (2007) PLoS Biol. 5:E327-E327.