产品名称：Anti-AKAP5/FITC抗体

Anti-AKAP5/FITC抗体

产品编号YB-6980R-FITC

英文名称Anti-AKAP5/FITC

中文名称FITC标记的A激酶锚定蛋白5抗体

别    名AKAP 150;A kinase (PRKA) anchor protein 5; A kinase anchor protein 5; A kinase anchor protein 79kDa; A kinase anchoring protein 75/79; A-kinase anchor protein 5; A-kinase anchor protein 79 kDa; AKAP 5; AKAP 75; AKAP 79; AKAP-5; Akap5; AKAP5_HUMAN; AKAP75; AKAP79; cAMP dependent protein kinase regulatory subunit II high affinity binding protein; cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein; H21.

Anti-AKAP5/FITC抗体

说 明 书100ul  

研究领域细胞生物  **学  神经生物学  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,

产品应用IF=1:50-200  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量47/79(human/rat, mo)kDa

细胞定位细胞膜

性    状Lyophilized or Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human AKAP5

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Anti-AKAP5/FITC抗体

产品介绍background:

The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates (1). Specificity of PKA signaling is thought to be mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins. This family of proteins has been designated A-kinase anchoring proteins (AKAP) (1-3). Members of this family, including MAP2 (microtubule-associated protein 2), neuronally expressed AKAP 79 and AKAP 150, and the DNA binding AKAP 95, display differential tissue specificity and localization (4-6). Evidence suggests that AKAP 79 and AKAP 150 are both capable of anchoring PKA to postsynaptic densities (PSD), which are a network of proteins located on the internal surfaces of excitatory synapses。

Function:

May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. Anti-AKAP5/FITC抗体Association with to the beta2-adrenergic receptor (beta2-AR) not only regulates beta2-AR signaling pathway, but also the activation by PKA by switching off the beta2-AR signaling cascade.

Subunit:

Binding protein for dimer of the RII-beta regulatory subunit of cAMP-dependent protein kinase (PKA) and also for the protein kinase C (PKC) and the phosphatase calcineurin (PP2B). Each enzyme is inhibited when bound to the anchoring protein. Also binds the beta2-adrenergic receptor. Part of a complex containing AKAP5, ADCY5, ADCY6 AND PDE4C (By similarity). Interacts with ADCY8, and enhances its phosphorylation at lipid rafts.

Subcellular Location:

Membrane; Lipid-anchor.

Tissue Specificity:

Predominantly in the cerebral cortex and the postsynaptic densities of the forebrain, and to a lesser extent in adrenal medulla, lung and anterior Anti-AKAP5/FITC抗体pituitary.

Post-translational modifications:

Palmitoylation at Cys-36 and Cys-129 plays a key role in targeting to lipid rafts.

Similarity:

Contains 1 AKAP domain.

Database links:

UniProtKB/Swiss-Prot: P24588.3

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.