凋亡蛋白活性因子-1抗体
规格：1mg/1ml
英文名： APAF1(NT)
别名： APAF 1; APAF1; APAF-1; Apoptotic peptidase activating factor 1; Apoptotic protease activating factor; CED 4; CED4; KIAA0413; APAF_HUMAN; Apoptotic protease-activating factor 1.
分子量： 137kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human Apaf-1
交叉反应：Human, Mouse, Rat, Chicken, Dog, Cow, Horse,
凋亡蛋白活性因子-1抗体细胞定位：细胞浆
产品介绍：background: This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain(NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]. Function: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation 凋亡蛋白活性因子-1抗体of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Subunit: Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). Subcellular Location: Cytoplasm. Tissue Specificity: Ubiquitous. Highest levels of expression in ***** spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. Similarity: Contains 1 CARD domain. Contains 1凋亡蛋白活性因子-1抗体 NB-ARC domain. Contains 13 WD repeats. Gene ID: 317 Database links: Entrez Gene: 317 Human Entrez Gene: 11783 Mouse Entrez Gene: 78963 Rat Omim: 602233 Human SwissProt: O14727 Human SwissProt: O88879 Mouse SwissProt: Q9EPV5 Rat Unigene: 728891 Human Unigene: 220289 Mouse Unigene: 64522 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Apaf-1 （Apoptosis protease activating factor-1）调节细胞色素C依赖的Caspase-9原的自动催化活性，导致Caspase-3 的激活和引起凋亡。 Apaf-1在成年人的脾脏、外周血白细胞、肾脏、肺和胎儿脑、肾、肺中高水平表达。
凋亡蛋白活性因子-1抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 细胞生物 神经生物学 信号转导 细胞凋亡
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid