单甲基化组蛋白H3K7
规格：1mg/1ml
英文名： Histone H3 (mono methyl K79)
别名： Histone H3(mono methyl Lys79); mono methyl Histone H3(Lys79); mono methyl Histone H3(K79); H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene; Histone H3; H3S; Histone H3-I/
分子量： 15kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated Synthesised metylpeptide derived from human H
交叉反应：Human,
细胞定位：细胞核
单甲基化组蛋白H3K7产品介绍：background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3 is deposited into chromatin exclusively through a DNA replication-coupled pathway that can be associated with either DNA duplication or DNA repair synthesis during meiotic homologous recombination. Subunit: he nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Subcellular Location: Nucleus. Chromosome. Note=Localizes to both the large, transcriptionally active, somatic macronucleus (MAC) and the small, transcriptionally inert, germ line micronucleus (MIC). Post-translational modifications: Phosphorylated to form H3S10ph. H3S10ph promotes subsequent H3K14ac formation by GCN5. H3S10ph is only found in the mitotically dividing MIC, but not in the amitotically dividing MAC. H3S10ph is correlated with chromosome condensation during mitotic or meiotic micronuclear divisions.单甲基化组蛋白H3K7 Acetylation of histone H3 leads to transcriptional activation. H3K14ac formation by GCN5 is promoted by H3S10ph. H3K9acK14ac is the preferred acetylated form of newly synthesized H3. Acetylation occurs almost exclusively in the MAC. Methylated to form H3K4me. H3K4me is only found in the transcriptionally active MAC. Methylated to form H3K9me in developing MACs during conjugation, when genome-wide DNA elimination occurs. At this stage, H3K9me specifically occurs on DNA sequences being eliminated (IES), probably targeted by small scan RNAs (scnRNAs) bound to IES, and is required for efficient IES elimination. H3K9me is required for the interaction with the chromodomains of PDD1 and PDD3. The full-length protein H3S (slow migrating) is converted to H3F (fast migrating) by proteolytic removal of the first 6 residues. H3F is unique to MIC, and processing seems to occur regularly each generation at a specific point in the cell cycle. Similarity: Belongs to the histone H3 family. Gene ID: 8350 Database links: Entrez单甲基化组蛋白H3K7 Gene: 8350?/span>Human Entrez Gene: 8351?/span>Human Entrez Gene: 8352?/span>Human Entrez Gene: 8353?/span>Human Entrez Gene: 8354?/span>Human Entrez Gene: 8355?/span>Human Entrez Gene: 8356?/span>Human Entrez Gene: 8357?/span>Human Entrez Gene: 8358?/span>Human Entrez Gene: 8968?/span>Human Entrez Gene: 319152?/span>Mouse Entrez Gene: 319153?/span>Mouse Entrez Gene: 360198?/span>Mouse Entrez Gene: 97908?/span>Mouse Entrez Gene: 100364501?/span>Rat Entrez Gene: 100365669?/span>Rat Entrez Gene: 291159?/span>Rat Entrez Gene: 679994?/span>Rat Entrez Gene: 680511?/span>Rat Entrez Gene: 682330?/span>Rat Omim: 601128?/span>Human SwissProt: P68431?/span>Human SwissProt: Q16695?/span>Human SwissProt: Q93081?/span>Human SwissProt: P68433?/span>Mouse SwissProt: Q6LED0?/span>Rat Unigene: 132854?/span>Human Unigene: 247813?/span>Human Unigene: 247814?/span>Human Unigene: 248176?/span>Human Unigene: 443021?/span>Human Unigene: 484990?/span>Human Unigene: 532144?/span>Human Unigene: 533292?/span>Human Unigene: 546315?/span>Human Unigene: 586261?/span>Human Unigene: 591778?/span>Human Unigene: 221301?/span>Mouse Unigene: 261657?/span>Mouse Unigene: 377874?/span>Mouse Unigene: 390558?/span>Mouse Unigene: 397328?/span>Mouse Unigene: 138090?/span>Rat? Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
单甲基化组蛋白H3K7产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 **学 表观遗传学
储存条件： Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid