组蛋白赖氨酸去甲基化酶PHF8抗体
规格：1mg/1ml
英文名： PHF8
别名： PHD finger protein 8; PHD finger protein 8; Histone lysine demethylase PHF8; PHD finger protein 8; PHF8; PHF8_HUMAN; ZNF422.
分子量： 118kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human PHF8
交叉反应：Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
细胞定位：细胞核
组蛋白赖氨酸去甲基化酶PHF8抗体产品介绍：background: The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. Defects in this gene are a cause of mental retardation syndromic X-linked Siderius type (MRXSSD). Four transcript variants encoding different isoforms have been found for this gene. Function: Histone lysine demethylase with selectivity for thedi-and monomethyl states that plays a key role cell cycleprogression, rDNA transcription and brain development. Demethylatesmono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 andH3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylatedhistone H4 'Lys-20' residue (H4K20Me1). Acts as a transcriptionactivator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigeneticrepressive marks. Involved in cell cycle progression by beingrequired to control G1-S transition. Acts as a coactivator of rDNAtranscription, by activating polymerase I (pol I) mediatedtranscription of rRNA genes. Required for brain development,probably by regulating expression of neuron-specific genes. Onlyhas activity toward H4K20Me1 when nucleosome is used as a substrateand when not histone octamer is used as substrate. May also haveweak activity toward dimethylated H3 'Lys-36' (H3K36Me2), 组蛋白赖氨酸去甲基化酶PHF8抗体however,the relevance of this result remains unsure in vivo. Specificallybinds trimethylated 'Lys-4' of histone H3 (H3K4me3), affectinghistone demethylase specificity: has weak activity toward H3K9Me2in absence of H3K4me3, while it has high activity toward H3K9me2when binding H3K4me3. Subunit: Interacts with POLR1B, UBTF, SETD1A, HCFC1, E2F1 andZNF711. Subcellular Location: Nucleus. Nucleus, nucleolus. Note=Recruitedto H3K4me3 sites on chromatin during interphase. Dissociates fromchromatin when cells enter mitosis. Post-translational modifications: Phosphorylation at Ser-69 and Ser-120 are required fordissociation from chromatin and accumulation of H4K20Me1 levelsduring prophase.组蛋白赖氨酸去甲基化酶PHF8抗体 DISEASE: Defects in PHF8 are the cause of mental retardationsyndromic X-linked Siderius type (MRXSSD) [MIM:300263]. A disordercharacterized by mild to borderline mental retardation with orwithout cleft lip/cleft palate. Similarity: Belongs to the JHDM1 histone demethylase family.JHDM1D subfamily. Contains 1 JmjC domain. Contains 1 PHD-type zinc finger. Database links: UniProtKB/Swiss-Prot: Q9UPP1.3 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产组蛋白赖氨酸去甲基化酶PHF8抗体品应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-400 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 **学 表观遗传学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid