Caspase-3抗体

Caspase-3抗体经ICC, IHC, WB, FACS严格验证,品质保证.广泛种属反应性附有用户反馈信息.确保特异性!**的技术支持帮您更快得到实验结果，现货.我司提供8万种科研用专业抗体,经严格质控,特异性高,可重复性强,验证数..可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。

产品编号bs-0081R

英文名称Caspase-3

中文名称活化半胱胺酸蛋白酶蛋白-3抗体

别    名Caspase-3 subunit p17; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1.  

JournalPMIDIFApplicationBrain (2013)241546179.9150IHCBiomaterials (2013)238100817.6040WBMolecular Neurobiology (2014)245102755.4710IFJournal of Cellular and Molecular Medicine (2013)234807864.7530WBOsteoarthritis and Cartilage (2012)229357884.2620WBJournal of Cellular Physiology (2013)242651224.2180IFInternational Journal of Nanomedicine (2015)258482614.1950ICCApoptosis (2013)230540843.9490IHC-PEuropean Journal of Nutrition (2015)259309653.8400IHC-PNanotechnology (2015)266836603.8210ICCInternational Journal of Pharmaceutics (2014)25218185 3.7850 IHC-PCell and Tissue Research (2014)253583983.5650IHC-PPLoS One (2014) 25506932 3.5340 WB European Journal of Nutrition (2016) 26907089 3.4670 IHC-P American Journal of Translational Research (2016) PMC4846955 3.4020 WB International Journal of Biological Sciences (2014) 24719562 3.1680 WB Journal of Cellular Biochemistry (2013) 23553804 3.0620 WB Toxicology in Vitro (2016) 27216471 2.9000 ELISA Journal of Ethnopharmacology (2014) 24583069 2.7550 WB Life Sciences (2015) 26546416 2.7020 WB Nutrition and Cancer (2014) 24660968 2.6950 WB Histochemistry and cell biology (2012) 22585037 2.6130 IHC-P/WB Toxicology and Applied Pharmacology (2016) 27105818 2.5320 WB Tumor Biology (2014) 24839008 2.5180 WB Biochemical and Biophysical Research Communications (2013) 24184479 2.4060 WB Cell Biochemistry and Biophysics (2014) 25260394 2.3800 WB Cell Biochemistry and Biophysics (2014) 25260396 2.3800 WB World J Gastroenterol (2015) 26379399 2.3690 WB OncoTargets and Therapy (2015) 26677335 2.3110 ICC Cell Proliferation (2013) 23510477 2.2650 WB Journal of Pharmacological Sciences (2014) 24942213 2.1500 WB Molecules (2014) 25123188 2.0950 Other Neuroscience Letters (2012) 23123774 2.0260 IHC Cell Biochemistry and Function (2013) 23508548 1.8540 ICC Molecular Medicine Reports (2015) 26165988 1.5540 FCMBrazilian Journal of Medical and Biological Research (2014)255179181.1390WB

规格价格50ul/780元 购买    100ul/1280元 购买    200ul/1980元 购买    大包装/询价

说 明 书50ul  100ul  200ul

研究领域肿瘤  细胞生物  神经生物学  信号转导  细胞凋亡  Alzheimer's  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,

Caspase-3抗体产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=2μg/Test IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量177kDa

细胞定位细胞浆

性    状Lyophilized or Liquid

浓    度1mg/1ml

免 疫 原KLH conjugated synthetic peptide derived from human Nestin

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

Caspase-3抗体产品介绍background:

Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.

Function:

Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Subunit:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.

Subcellular Location:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3

Tissue Specificity:

CNS stem cells.

Post-translational modifications:

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.

Similarity:

Belongs to the intermediate filament family.

SWISS:

P48681

Gene ID:

10763

Database links:

Caspase-3抗体溶解方法：

1、在我们公司购买抗体后，查看标签上的规格，如：0.1ml、0.2ml

2、 以规格为0.1ml的冻干粉抗体为例（部分抗体为液体，无需再溶解）：

溶解方法一：用移液器加0.1ml双蒸水到试管内，混匀，-20℃保存；

若为其它规格（如：0.2ml）的冻干粉抗体，则加与标签上规格一致的（如：0.2ml）双蒸水溶解，混匀，-20℃保存。

溶解方法二：用移液器加 0.05ml（规格的 50%）双蒸水到试管内，再加 0.05ml（规格的 50%）

的甘油到试管内，混匀，-20℃保存； 若为其它规格（如：0.2ml）的冻干粉抗体，则加标签上规格的 50%（如：0.1ml）双蒸水，再加标签上规格的50%（如：0.1ml）甘油溶解，混匀，-20℃保存。

3、抗体的分装

抗体溶解后，将其分装成 5-10支（以0.1mlCaspase-3抗体为例，20ulx5支或 10ulx10支），-20℃保存，用一支取一支，避免反复冻溶。

另：预试验可做几个梯度1：100、1：200、1：400稀释，如果背景高，还可再稀释，选一个*佳的稀释比例，再正式做，效果*好。工作液可用“抗体稀释液”稀释。

后续试验时，再使用对应的缓冲液稀释成工作液，即用即配。

我们的抗体（冻干粉），在常温放置下，至少一个月有效；若在-20℃下保存（推荐），至少三年有效。

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