LOCUS Exported 4053 bp ds-DNA circular SYN 01-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..4053
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 439..784
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 823..1533
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
terminator 1550..1802
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
rep_origin complement(2234..2822)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2993..3853)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3854..3958)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctttcaaag atacagtctc
421 agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg
481 attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc
541 ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag
601 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
661 cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca
721 atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag
781 aacacggggg actctagagg atccccgggt ggtcagtccc ttatggtgag caagggcgag
841 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
901 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
961 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
1021 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
1081 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
1141 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
1201 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
1261 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
1321 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
1381 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
1441 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
1501 tccaccggcg gcatggacga gctgtacaag tgagagctcg aatttccccg atcgttcaaa
1561 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
1621 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
1681 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
1741 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
1801 tcggggtacc gagctcgaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
1861 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
1921 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
1981 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
2041 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
2101 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
2161 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
2221 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
2281 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
2341 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
2401 tcccttcggg aagcgtggcg ctttctcaaa gctcacgctg taggtatctc agttcggtgt
2461 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
2521 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
2581 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
2641 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
2701 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
2761 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
2821 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
2881 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
2941 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
3001 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
3061 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
3121 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
3181 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
3241 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
3301 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
3361 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
3421 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
3481 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
3541 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
3601 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
3661 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
3721 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
3781 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
3841 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
3901 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
3961 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
4021 ataaaaatag gcgtatcacg aggccctttc gtc
//