LOCUS Exported 4031 bp ds-DNA circular SYN 26-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4031)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 26, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4031
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 413..1158
/label=3×CaMV 35S promoter
misc_feature 1299..1355
/label=MCS
/note="multiple cloning site"
misc_feature 1356..1567
/label=cEYFP
misc_feature 1574..1784
/label=CaMV 35S terminator
rep_origin complement(2212..2800)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2971..3831)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3832..3936)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccgg cgcgccaccg gtcaacatgt
421 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag
481 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc
541 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca
601 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga
661 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
721 gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat
781 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc
841 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga
901 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga
961 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
1021 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
1081 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
1141 atttcatttg gagaggacgt cgagagttct caacacaaca tatacaaaac aaacgaatct
1201 caagcaatca agcattctac ttctattgca gcaatttaaa tcatttcttt taaagcaaaa
1261 gcaattttct gaaaattttc accatttacg aacgatagag atctcgagct caagcttcga
1321 attctgcagt cgacggtacc gcgggcccgg gatcctgggc agcgtgcagc tcgccgacca
1381 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
1441 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
1501 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtgaagatc
1561 cacctagtct agagtccgca aaaatcacca gtctctctct acaaatctat ctctctctat
1621 ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt
1681 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
1741 tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacgcggcc gcggcgcgcc
1801 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
1861 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
1921 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
1981 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
2041 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
2101 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
2161 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
2221 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
2281 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
2341 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
2401 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
2461 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
2521 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
2581 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
2641 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
2701 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
2761 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
2821 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
2881 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
2941 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
3001 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
3061 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
3121 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
3181 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
3241 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
3301 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
3361 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
3421 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
3481 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
3541 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
3601 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
3661 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
3721 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
3781 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
3841 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
3901 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
3961 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
4021 gccctttcgt c
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