LOCUS Exported 4402 bp ds-DNA circular SYN 26-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4402)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 26, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4402
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 435..1319
/label=2X35S promoter + TL enhancer
CDS 1323..1841
/codon_start=1
/product="N-terminal fragment of mVenus for use in
bimolecular fluorescence complementation (BiFC) (Kodama and
Hu, 2010)"
/label=n(1-174)EYFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIE"
misc_feature 1845..1910
/label=MCS
/note="multiple cloning site"
misc_feature 1923..2133
/label=35S terminator
rep_origin complement(2583..3171)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3342..4202)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4203..4307)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct
421 gtttgccaac cggtcaacat gtggagcacg acacacttgt ctactccaaa aatatcaaag
481 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa
541 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg
601 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct
661 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
721 acgttccaac cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca
781 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
841 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc
901 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
961 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
1021 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
1081 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
1141 cttcctctat ataaggaagt tcatttcatt tggagaggac gtcgagagtt ctcaacacaa
1201 catatacaaa acaaacgaat ctcaagcaat caagcattct acttctattg cagcaattta
1261 aatcatttct tttaaagcaa aagcaatttt ctgaaaattt tcaccattta cgaacgatag
1321 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
1381 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
1441 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
1501 ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga
1561 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
1621 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
1681 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
1741 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
1801 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggactccgga ctcagatctc
1861 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc acctagtcta
1921 gagtccgcaa aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga
1981 ataatgtgtg agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt
2041 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
2101 aatttctaat tcctaaaacc aaaatccagt gacgcggccg cacccataat acccataata
2161 gctgtttgcc agtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
2221 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
2281 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
2341 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
2401 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
2461 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
2521 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
2581 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
2641 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
2701 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
2761 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
2821 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
2881 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
2941 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
3001 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
3061 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
3121 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
3181 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
3241 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
3301 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
3361 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
3421 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
3481 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
3541 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
3601 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
3661 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
3721 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
3781 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
3841 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
3901 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
3961 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
4021 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
4081 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
4141 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
4201 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
4261 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
4321 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
4381 cgtatcacga ggccctttcg tc
//